Poly dg dc

Manufactured by Thermo Fisher Scientific

Sourced in United States

Poly(dG:dC) is a synthetic DNA polymer composed of alternating guanine (dG) and cytosine (dC) nucleotides. It is a commonly used laboratory reagent for various applications in molecular biology and biochemistry.

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Lab products found in correlation

Poly dg dc, by InvivoGen (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Tlrl pgcn, by InvivoGen (1 mentions) Tlrl isdn, by InvivoGen (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Nucleofector device, by Lonza (1 mentions) Cell line kit 5, by Lonza (1 mentions) Poly da dt, by Merck Group (1 mentions)

2 protocols using poly dg dc

Type-I IFN Activation in Macrophages

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Interferon stimulating DNA (ISD) and Poly(dG:dC) were obtained from InvivoGen (tlrl-pgcn, tlrl-isdn). To activate type-I IFN response in macrophages, ISD or Poly(dG:dC) were mixed with Lipofectamine 3000 (Invitrogen, L3000150) at a ratio of 1:1 (v/w), and then added to cells at a final concentration of 1 μg/ml.

Mirji G., Worth A., Bhat S.A., Sayed M.E., Kannan T., Goldman A.R., Tang H.Y., Liu Q., Auslander N., Dang C.V., Abdel-Mohsen M., Kossenkov A., Stanger B.Z, & Shinde R.S. (2022). The microbiome-derived metabolite TMAO drives immune activation and boosts responses to immune checkpoint blockade in pancreatic cancer. Science immunology, 7(75), eabn0704.

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Nucleofection of Immune Cells

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The nucleofector device (Amaxa, Germany) and cell line kit V (Amaxa Biosystems, USA) were used to transfect poly(dA:dT) (Sigma, USA), poly(dG:dC) (Invivogen, USA) or ISD (Invivogen, USA) into BJAB and Jurkat cells. Transfection efficiency, as determined by green fluorescent protein expression in parallel plates, varied between 50% and 60%. Transfection of HeLa, MSCs and HEK293T cells with poly(dA:dT), poly(dG:dC) or ISD was done using Lipofectamine 2000 (Invitrogen, USA) according to standard procedures.

Dong G., You M., Ding L., Fan H., Liu F., Ren D, & Hou Y. (2015). STING Negatively Regulates Double-Stranded DNA-Activated JAK1-STAT1 Signaling via SHP-1/2 in B Cells. Molecules and Cells, 38(5), 441-451.

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