Necrosulfonamide

Manufactured by Selleck Chemicals

Sourced in United States, China

Necrosulfonamide is a chemical compound used in laboratory research. It is a sulfur-containing compound that can be used in various applications within a controlled laboratory setting. The core function of Necrosulfonamide is to serve as a research tool for scientific investigations, without making claims about its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Necrosulfonamide (S8251) (4 citations) Necrosulfonamide (NSA, (2 citations)

27 protocols using necrosulfonamide

Culturing A549 and Small Airway Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549 cells (American Type Culture Collection, #CCL-185) were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS). The cells were passaged <20 times. Small airway epithelial cells (American Type Culture Collection, #PCS-301-010) were cultured in small airway epithelial growth media (Lonza #CC-3118) and passaged < 7 times. For studies using cell death inhibitors, deferoxamine mesylate (Selleckchem #S5742), necrosulfonamide (Selleckchem #S8251), and Z-VAD-FMK (Selleckchem #S7023) were dissolved in DMSO, and cells were treated at indicated concentrations

Sauler M., McDonough J.E., Adams T.S., Kothapalli N., Barnthaler T., Werder R.B., Schupp J.C., Nouws J., Robertson M.J., Coarfa C., Yang T., Chioccioli M., Omote N., Cosme C J.r., Poli S., Ayaub E.A., Chu S.G., Jensen K.H., Gomez J.L., Britto C.J., Raredon M.S., Niklason L.E., Wilson A.A., Timshel P.N., Kaminski N, & Rosas I.O. (2022). Characterization of the COPD alveolar niche using single-cell RNA sequencing. Nature Communications, 13, 494.

+ Open protocol

+ Expand

Ferroptosis and Necroptosis Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vemurafenib (cat No. S1267), sorafenib (cat No. S1040), ferroptosis inhibitor Ferrostatin-1 (Fer-1) (cat no. S7243), caspase inhibitor Z-VAD-FMK (cat No. S7023) and MLKL inhibitor Necrosulfonamide (cat No. S8251) were purchased from Selleck (Shanghai, China).

Tang F., Li S., Liu D., Chen J, & Han C. (2020). Sorafenib sensitizes melanoma cells to vemurafenib through ferroptosis. Translational Cancer Research, 9(3), 1584-1593.

+ Open protocol

+ Expand

Caco-2 Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caco-2 cells seeded on a 96-well plate were stimulated by inhibitors added as follows. After treatment Monmouthfor 48 h, the MTT experiment was carried out to determine the cell viability. The working concentrations of Vitamin E, Z-VAD-FMK, 3-Methyladenine, VX-765, GSK-872 (MedChemExpress, Monmouth, NJ, USA), Necrosulfonamide and Nec-1s (Selleck, Houston Texas USA) were 30, 10, 10, 5, 10, 0.5, and 10 μM, respectively. The inhibitors were dissolved in DMEM (1% BSA + 0.1% DMSO) and combined with the CLNAs at their IC₅₀.

Ren Q., Yang B., Zhu G., Wang S., Fu C., Zhang H., Ross R.P., Stanton C., Chen H, & Chen W. (2020). Antiproliferation Activity and Mechanism of c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 on Colon Cancer Cells. Molecules, 25(5), 1225.

+ Open protocol

+ Expand

Dissecting Cell Death Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorafenib (Bay 43-9006, Sigma-Aldrich), 17-AAG (Tanespimycin, MCE, HSP90α inhibitor), Z-VAD-FMK (Selleck, Caspase Inhibitor), Necrostatin-1 (Selleck, RIPK1 inhibitor), Necrosulfonamide (Selleck, MLKL inhibitor).
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazoliumbromide) was obtained from Fluka Chemical Corp (Ronkonkoma, NY, USA) and was dissolved in 0.01 M phosphate-buffered saline (PBS). Antibodies against RIPK1(A7414), RIPK3(A5431), MLKL(A5579), HIF-1α(A16873) were purchased from Abclonal Technology (Wuhan, China). Antibodies against, HSP90α(BS6461), were products of Bioworld Technology (USA). Antibodies against AFP (4448) and LAMP2 (49067) were products of Cell Signaling Technology (Beverly, MA, USA). Antibodies against Lamin A (sc-293162), β-Tubulin (sc-166729) and β-Actin (sc-8432) were products of Santa Cruz Biotechnology (USA). Normal mouse and rabbit IgG-HRB secondary antibodies were purchased from Santa Cruz Biotechnology.

Liao Y., Yang Y., Pan D., Ding Y., Zhang H., Ye Y., Li J, & Zhao L. (2021). HSP90α Mediates Sorafenib Resistance in Human Hepatocellular Carcinoma by Necroptosis Inhibition under Hypoxia. Cancers, 13(2), 243.

+ Open protocol

+ Expand

Comprehensive Ferroptosis and Necroptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Erastin (S7242), RSL3 (S8155), liproxstatin-1 (S7699), Z-VAD-FMK (S7023), FIN56 (S8254), necrosulfonamide (S8251), CCT137690 (S2744), oxaliplatin (S1224), BMS-345541 (S8044), carboplatin (S1215), etoposide (S1225), vincristine (S9555), deferoxamine (S5685), chloroquine (S6999), and 4OI (S5929) were purchased from Selleck Chemicals. The antibodies to SQSTM1 (5114), ATG5 (2630), MLKL (14993), NCOA4 (66849), FTH1 (3998), ATG7 (2631), SLC7A11 (12691), and ACTB (3700) were purchased from Cell Signaling Technology. The antibodies to MAP1LC3B (NB100-2220), RIPK1 (NBP1-77077), and RIPK3 (NBP1-77299) were purchased from NOVUS. The antibody to GPX4 (ab125066) was purchased from Abcam.

Liu K., Huang J., Liu J., Klionsky D.J., Kang R, & Tang D. (2022). Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cells. Cell Death & Disease, 13(6), 521.

+ Open protocol

+ Expand

Daurisoline, Apoptosis, and Necroptosis Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Daurisoline (DAS) was purchased from MCE (MedChemExpress, Shanghai, China). DAS was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, Germany) and stored at −80°C for the in vitro study. For the in vivo study, DAS was firstly dissolved in 50% PEG300 and then in 50% saline (MCE, Shanghai, China). Z-VAD-FMK (Z-VAD) was purchased from MCE. Necrostatin-1 (Nec-1), necrosulfonamide (NSA), and ferrostatin-1 (Fer-1) were purchased from Selleck (Selleck, Shanghai, China). N-acetylcysteine (NAC) was purchased from MCE.

Yuan S., Pan Y., Xu T., Zhang L., Chen X., Wang F., Liu Q, & Jia L. (2022). Daurisoline Inhibits ESCC by Inducing G1 Cell Cycle Arrest and Activating ER Stress to Trigger Noxa-Dependent Intrinsic and CHOP-DR5-Dependent Extrinsic Apoptosis via p-eIF2α-ATF4 Axis. Oxidative Medicine and Cellular Longevity, 2022, 5382263.

+ Open protocol

+ Expand

Cytotoxicity Assay with Diverse Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tolinapant was obtained from Astex Pharmaceuticals (Cambridge, UK). Entinostat, necrosulfonamide and emricasan (IDN-6556) were supplied by Selleckchem (Houston,TX). Z-VAD-fmk was purchased from Calbiochem (Gibbstown, NJ). TNFα was supplied by Prospec (Rehovot, Israel). 5FU and oxaliplatin were obtained from Belfast City Hospital Pharmacy.

Crawford N., Stott K.J., Sessler T., McCann C., McDaid W., Lees A., Latimer C., Fox J.P., Munck J.M., Smyth T., Shah A., Martins V., Lawler M., Dunne P.D., Kerr E.M., McDade S.S., Coyle V.M, & Longley D.B. (2021). Clinical Positioning of the IAP Antagonist Tolinapant (ASTX660) in Colorectal Cancer. Molecular Cancer Therapeutics, 20(9), 1627-1639.

+ Open protocol

+ Expand

Ferroptosis Inducers and Inhibitors Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liproxstatin-1, Z-VAD-FMK, necrostatin-1, erastin, (1S,3R)-RSL3 (RSL3), ferroptosis inducer (FIN56), 20S proteasome inhibitor, cycloheximide, deferoxamine, eugenol, and necrosulfonamide were obtained from Selleck Chemicals. Rotenone, diethyl butylmalonate, antimycin, and NaN₃ were purchased from Santa Cruz Biotechnology. Anti-DACH1 antibody, anti-p53 antibody, anti-p53 (phospho S392) antibody, anti-SLC25A37 antibody, anti-Hsp90 antibody, anti-Voltage dependent anion channel protein antibody, anti-lamin B antibody, and anti-beta actin antibody were obtained from Abcam Technology. Anti-mouse IgG and anti-rabbit IgG were bought from Cell Signaling Technology.

Guo M., Zhuang Y., Wu Y., Zhang C., Cheng X., Xu D, & Zhang Z. (2024). The cell fate regulator DACH1 modulates ferroptosis through affecting P53/SLC25A37 signaling in fibrotic disease. Hepatology Communications, 8(3), e0396.

+ Open protocol

+ Expand

Antibody and Inhibitor Sources for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies to MAP1LC3B (#3868), actin (#3700), c-CASP8 (#8592 and #9496), C-CASP9 (#9505 and #9509), BAD (#9268), BBC3 (#4976), BAX (#2772), BCL2L1 (#2764), BCL2 (#2872), C-CASP3 (#9664), JNK (#9252), p-JNK (#9255), ERK (#4695), p-ERK (#4370), p38 (#8690), p-p38 (#4511), JUN (#9165), and p-JUN (#3270) were obtained from Cell Signaling Technology (Danvers, MA, USA). Monoclonal anti-HMGB1 neutralizing antibody (clone 2G7) was a gift from Dr. Kevin Tracey. Monoclonal anti-TFAM neutralizing antibody (clone F-6; #sc-166965) was obtained from Santa Cruz Biotechnology (Dallas, Texas, USA). Monoclonal anti-AGER neutralizing antibody (clone 697023; #MAB11795) was obtained from R&D System (Minneapolis, MN, USA). Spautin-1 (#S7888), 3-methyladenine (#S2767), LY294002 (#S1105), chloroquine (#S4157), bafilomycin A1 (#S1413), oxaliplatin (#S1224), Z-VAD-FMK (#S7023), necrostatin-1 (#S8037), necrosulfonamide (#S8251), ferrostatin-1 (#S7234), liproxstatin-1 (#S7699), SP600125 (#S1460), SB203580 (#S1076), SB239063 (#S7741), SCH772984 (#S7101), and LY3214996 (#S8534) were obtained from Selleck Chemicals (Houston, TX, USA). 5-fluorouracil (#F6627), TRAIL (#T5694), Mito-TEMPO (#SML0737), and CC-401 (#SML1613) were obtained from Sigma (St. Louis, MO, USA).

Yang M., Li C., Zhu S., Cao L., Kroemer G., Zeh H., Tang D, & Kang R. (2018). TFAM is a novel mediator of immunogenic cancer cell death. Oncoimmunology, 7(6), e1431086.

+ Open protocol

+ Expand

Investigating TNFα-Mediated Cell Death

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant TNFα (R&D Systems, Cat#210-TA-005); Recombinant mouse mTNFα (Cell sciences, Cat# CRT192C); Cycloheximide (Sigma, Cat# C-6255); SM-164 (Selleckchem, custom-synthesized); Birinapant (Selleck Chemicals, Cat# S7015); zVAD (Selleck Chemicals, Cat# S8102); Nec-1s (7-Cl-O-Nec-1) (Selleck Chemicals, Cat# S8641); GSK-872 (Sigma, Cat# 5303890001); Necrosulfonamide (Sigma, 480073); LPS (Sigma, Cat# L4391); IDN-6556 (Selleck Chemicals, Cat# S7775); Poly(I:C) (InvivoGen, Cat# tlrl-pic-5); 5Z-7-Oxozeaenol (Sigma, Cat# O9890); 5-fluorouracil (Selleck Chemicals, Cat# S1209); Necrosulfonamide (Selleck Chemicals, Cat# S825); and ELISA Kit for Human TNFα (BioLegend, Cat# 430206).

Cai J., Hu D., Sakya J., Sun T., Wang D., Wang L., Mao X, & Su Z. (2021). ABIN-1 is a key regulator in RIPK1-dependent apoptosis (RDA) and necroptosis, and ABIN-1 deficiency potentiates necroptosis-based cancer therapy in colorectal cancer. Cell Death & Disease, 12(2), 140.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!