Biotinylated miR-641 and the corresponding mutant/negative control were synthesized and purchased from GenePharma (Shanghai, China). The oligonucleotides were transfected into U87 and LN229 cells using Lipofectamine 3000 (Invitrogen). Forty-eight hours later, the cell lysates were incubated with M-280 streptavidin magnetic beads (Invitrogen, Cat. No. 20164). qRT-PCR was used to detect the expression of COX10-AS1.
Biotinylated mir 641
Manufactured by GenePharma
Sourced in China
Biotinylated miR-641 is a synthetic oligonucleotide molecule that is conjugated with biotin. This product is intended for use in various research applications, such as study of gene expression regulation, RNA-protein interactions, and molecular biology techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biotinylated mir 641
1
Biotinylated miR-641 pulldown assay
2
Biotinylated miR-641 Pulldown
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Biotinylated miR-641 and the corresponding mutant/negative control were synthesized and purchased from GenePharma (Shanghai, China). The oligonucleotides were transfected into U87 and LN229 cells using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours later, the cell lysates were incubated with M-280 streptavidin magnetic beads (Invitrogen, USA). qRT-PCR was used to detect the expression of COX10-AS1.
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