The principal components of bacterial culture: LB broth, tryptone, and yeast extract; antibiotics: kanamycin; protein expression and extraction reagents: IPTG, Tris–HCL and base, glycine, lysozyme, PMSF, SDS, acrylamide/bis-acrylamide, ammonium persulfate, tetramethylethylenediamine (TEMED), DTT, and EDTA, and Coomassie G-250 were purchased from Himedia and VWR life science. Inclusion body solubilizing and protein refolding reagents: guanidine hydrochloride, urea, arginine hydrochloride, and isopropanol were from Sigma-Aldrich. PCR cloning kit: pJET1.2; Q5 high fidelity DNA polymerase and restriction enzymes, T4-DNA ligase, and rapid protein assay BCA kit were from New England Biolabs (NEB) and Thermo Scientific. Protein purification was performed by AKTA FPLC systems using superose 12 10/300 size-exclusion column which were purchased from GE Healthcare. Surface Plasmon Resonance instrument (Autolab ESPRIT) and BLI systems (Octet RED96) were used to measure the binding affinity.
Ammonium persulfate
Manufactured by HiMedia
Sourced in India
Ammonium persulfate is a chemical compound that is commonly used as an oxidizing agent and initiator in various applications. It is a crystalline, white solid with a chemical formula of (NH4)2S2O8. Ammonium persulfate is known for its ability to initiate polymerization reactions and is often used in the production of acrylamide-based polymers and copolymers.
Automatically generated - may contain errors
Lab products found in correlation
Temed, by HiMedia (3 mentions)
Sodium dodecyl sulfate (sds), by HiMedia (3 mentions)
Glycine, by HiMedia (3 mentions)
Paraformaldehyde (pfa), by HiMedia (3 mentions)
Ethidium bromide, by HiMedia (3 mentions)
Sodium chloride (nacl), by HiMedia (2 mentions)
50 bp dna ladder, by Merck Group (2 mentions)
Transferrin a488, by Merck Group (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hoechst, by Merck Group (2 mentions)
Mowiol, by Merck Group (2 mentions)
Loading dye, by Merck Group (2 mentions)
Nuclease free water, by HiMedia (2 mentions)
Guanidine hydrochloride, by Merck Group (1 mentions)
Rapid protein assay bca kit, by New England Biolabs (1 mentions)
Akta fplc system, by GE Healthcare (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
9 protocols using ammonium persulfate
1
Bacterial Protein Expression and Purification
2
Antioxidant and Tyrosinase Inhibition Assays
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For the study, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), DMEM-Low glucose, DMEM-high glucose, MTT Reagent, Butylated hydroxytoluene (BHT), ascorbic acid and ammonium persulfate were purchased from Hi-Media Pvt. Ltd. (Mumbai, India). Mushroom tyrosinase and doxorubicin were procured from Sigma-Aldrich Chemicals Pvt. Ltd. (Bengaluru, India). L-DOPA and sodium phosphate were obtained from SRL Pvt. Ltd. (Mumbai, India). Kojic acid was ordered from Cayman chemicals (Ann Arbor, MI, USA). Methanol and DMSO used were of analytical grade.
3
Colorimetric Assay for Antioxidant Capacity
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The reagents hydrochloric acid (HCl), nickel chloride hexahydrate (NiCl2·6H2O), ferric chloride hexahydrate (FeCl3·6H2O), hydrogen peroxide (H2O2), urea, glucose, fructose, glycine, aspartic acid, 3,5,3,5-tetramethylbenzidine (TMB), ammonium persulfate (APS), aniline, l -cystine, and also the chemicals for the phosphate and sodium buffer preparations were procured from Himedia (P) Ltd., India. Commercially available orange juices were used for the real sample analyses. All the reagents were used as received without any further purification.
4
Adsorption of Lead from Aqueous Solutions
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Commercially available bentonite clay, ammonium persulfate, N-acetyl-N,N,N-trimethyl ammonium bromide (CTAB) and sodium dodecyl sulfate were procured from HiMedia. The adsorbents used in the study were Nanoclay, hydrogel and hydrogel composite, which were prepared in the laboratory. Lead, nitric acid, hydrochloric acid and acrylic acid used for hydrogel preparation were obtained from Finar. Millipore distilled water was used for preparation of synthetic lead solution in different concentrations and also for all the other purposes. 5% Nitric acid was used for preparation of lead standard solution to plot standard curve for ICP analysis.
5
Anti-Apoptosis Signaling Pathway Assay
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o-Aminobenzenethiol
and salicylaldehyde were purchased from Aldrich. Sodium dodecyl sulfate
(SDS), Tris-base, glycine, sodium chloride, acrylamide/bis-acrylamide
30% solution, ammonium persulfate (APS), Bradford reagent, tetramethylethylenedi-amine
(TEMED), 5X Laemmli buffer, 2-mercaptoethanol, Poncheau-S stain, and a pre-stained protein marker were purchased from HiMedia.
Tween 20 and a protease inhibitor cocktail were purchased from Sigma.
An immobilon-P polyvinylidene fluoride (PVDF) membrane with a 0.45
μm pore size was purchased from Merck Millipore. Antibodies,
namely, anti-p53 (cat. no. 2527T), anti-caspase 3 (14220T), anti-beta
actin (4970T), anti-bax (5053T), and goat horseradish peroxidase-linked
anti-rabbit (7074P) were purchased from Cell Signaling Technology.
The Clarity western enhanced chemiluminescence (ECL) substrate was
purchased from Bio-Rad.
and salicylaldehyde were purchased from Aldrich. Sodium dodecyl sulfate
(SDS), Tris-base, glycine, sodium chloride, acrylamide/bis-acrylamide
30% solution, ammonium persulfate (APS), Bradford reagent, tetramethylethylenedi-amine
(TEMED), 5X Laemmli buffer, 2-mercaptoethanol, Poncheau-
Tween 20 and a protease inhibitor cocktail were purchased from Sigma.
An immobilon-P polyvinylidene fluoride (PVDF) membrane with a 0.45
μm pore size was purchased from Merck Millipore. Antibodies,
namely, anti-p53 (cat. no. 2527T), anti-caspase 3 (14220T), anti-beta
actin (4970T), anti-bax (5053T), and goat horseradish peroxidase-linked
anti-rabbit (7074P) were purchased from Cell Signaling Technology.
The Clarity western enhanced chemiluminescence (ECL) substrate was
purchased from Bio-Rad.
6
Pendimethalin Extraction and Analysis Protocol
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Pendimethalin was a generous gift from Rallis Agrochemicals India Ltd. (Mumbai, India). The 6-aminopendimethalin and 3,4-dimethyl 2,6-dinitroaniline standards were purchased from Sigma Chemical Co. (St. Louis, USA). Polyurethane foam and nylon meshes were purchased from Merck Specialities Private Limited (Mumbai, India). Acrylamide, bisAcrylamide, ammonium persulfate, sodium alginate (SA), nutrient agar, agar–agar, sodium chloride, and calcium chloride were purchased from HiMedia Laboratories (Mumbai, India). All other chemicals used were of analytical grade and available commercially.
7
DNA Nanostructure Synthesis Protocol
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Dulbecco's Modified Eagle Medium (DMEM) was purchased from Lonza. DMEM-F12, HAMS-F12, Fetal bovine serum (FBS), PenStrap, Trypsin-EDTA (0.25%), collagen were purchased from Gibco and Phosphate buffer saline (PBS) was purchased from HyClone. The sequences used for DNA nanostructure synthesis (Table 12345), 6X loading dye, 50 bp DNA ladder, mowiol, transferrin-A488, Cy5, Hoechst, Pitstop-2, Dynasore and Lactose were ordered from Sigma Aldrich. Nuclease free water, ammonium persulfate, ethidium bromide, TEMED, paraformaldehyde and adherent cell culture dishes were purchased from Himedia. Tris-Acetate EDTA (TAE), Acrylamide/bisacrylamide sol 30% were purchased from GeNei. Magnesium chloride was ordered from SRL, India. Galectin-3 was provided as a gift from Johannes team at Institut Curie, Paris.
8
Cellular Imaging and Protein Identification
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All oligonucleotides (SI Table 1), moviol, Triton-X, loading dye, Alexa Fluor (phalloidin) 488, moviol, poly l lysine, membrane glycoproteins (CD98 and CD147), transferrin 488, galectin, Hoechost (DAPI), Cholera toxin B-subunit (CTxB), were purchased from sigma Aldrich. Ammonium persulfate, ethidium bromide, triton X, Tetramethylethylenediamine (TEMED), Paraformaldehyde and cell culture dishes for adherent cells (treated surface) were procured from Himedia. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, trypsin-Ethylenediaminetetraacetic acid (EDTA) (0.25%), Collagen1 rat tail were purchased from Gibco. Tris-acetate-EDTA (TAE), Acrylamide/Bisacrylamide sol 30% (29:1 ratio for SDS PAGE) were procured from GeNei. Magnesium chloride, NaCl, KCl, Na 2 HPO 4 , and KH 2 PO 4 were purchased from SRL, India and Santa cruez biotech respectively.
9
Endothelial Cell Culture and DNA Nanostructures
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1. Materials: Human Umbilical Vein Endothelial Cells (HUVECs), Endothelial Cell Growth Basal Medium-2 (EBM) and EGM-2 SingleQuots TM Supplements were obtained from Lonza. Fetal bovine serum (FBS), PenStrap, Trypsin-EDTA (0.25%), collagen was acquired from Gibco. Phosphate buffer saline (PBS) was purchased from HyClone. The sequences used for DNA nanostructure synthesis (Table 1-4), 6X loading dye, 50 bp DNA ladder, mowiol, transferrin-A488, Hoechst and triton-X 100 were ordered from Sigma Aldrich. Nuclease free water, ammonium persulfate, ethidium bromide, TEMED, paraformaldehyde (37%) and adherent cell culture dishes were procured from Himedia. Tris-Acetate EDTA (TAE), Acrylamide/bisacrylamide sol 30% were purchased from GeNei. Magnesium chloride was ordered from SRL, India. Geltrex TM and CellMask TM were obtained from ThermoFischer. Matrigel and collagen were purchased from Corning.
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