Phoenix reveal oct software

Image-guided OCT (OCT 2 with Micron IV, Phoenix Research labs, USA) was employed to obtain the full-retinal cross-sectional scans 7 d after the experimental light exposure as previously described (Wu et al. 2020 (link), 2022 (link)). In brief, intraperitoneal injection of ketamine hydrochloride (82.5 mg/kg bw) and xylazine (8.25 mg/kg bw) was administered to induce anesthesia in the mice, followed by dilation of pupils using 1% tropicamide and OCT imaging. Five scans from the superior, central and inferior retinas were acquired and averaged automatically by the Phoenix Reveal OCT software (Phoenix Research labs, USA). The averaged scans were subjected to morphological evaluation of the retina. The thickness of the photoreceptor outer nuclear layer (ONL), inner segment (IS) and outer segment (OS) as well as the inner nuclear layer (INL) was measured using Insight Image Segmentation Software for the Phoenix OCT and Retinal Imaging System (Version 2.0.5490, Voxeleron LLC, USA).

Image-guided OCT (OCT 2 with Micron IV, Phoenix Research Labs, United States) was performed to obtain full-retinal scans in the live animals as previously described (Wu et al., 2020 (link)). Briefly, in preparation for the OCT imaging, anesthesia was induced by intraperitoneal injection of ketamine hydrochloride (82.5 mg/kg bw) and xylazine (8.25 mg/kg bw), which was followed by pupil dilation using 1% tropicamide (Santen Pharmaceutical, Japan). OCT scans acquired and averaged using Phoenix Reveal OCT Software (Phoenix Research Labs, United States) were then subjected to thickness measurement of the outer nuclear layer (ONL) using Insight Image Segmentation Software for the Phoenix OCT and Retinal Imaging System (Version 2.0.5490, Voxeleron LLC, United States).

Image-guided OCT (OCT 2 with Micron IV, Phoenix Research Labs, USA) was performed to scan the retina 7 d post illumination as previously described [10 (link)]. In brief, after administering anesthetic cocktail of ketamine hydrochloride (82.5 mg/kg body weight) and xylazine (8.25 mg/kg body weight), pupil dilation was induced using 1% tropicamide (Santen Pharmaceutical, Japan) to prepare the mice for the OCT imaging. Five full-retinal scans were obtained and automatically averaged using the Phoenix Reveal OCT software (Phoenix Research Labs, USA). The thickness of the ONL was measured using the Insight Image Segmentation Software, an adjunct software to the Phoenix OCT and Retinal Imaging System (Version 2.0.5490, Voxeleron LLC, USA).