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Superscript 3 with platinum

Manufactured by Thermo Fisher Scientific
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Sourced in United States

Superscript III with Platinum is a reverse transcriptase enzyme used for cDNA synthesis. It is designed for robust, sensitive, and specific reverse transcription of RNA templates.

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Lab products found in correlation

One step rt pcr system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

SuperscriptTM III One-Step with PlatinumTM Taq DNA Polymerase SuperScript III Platinum SYBR Green One-Step RTqPCR Kit with ROX SuperScript III Platinum Two-Step qRT-PCR Kit with SYBR Green SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq High Fidelity DNA Polymerase kit SuperScript® III Platinum One-Step Quantitative RT-PCR System with Rox SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity SuperScript III Platinum One-Step qRT-PCR Kit with ROX Superscript III One-Step RT-PCR with Platinum Taq kit SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase SuperScriptTM III One-Step PCR System with Platinum® Taq DNA Polymerase kit

2 protocols using superscript 3 with platinum

1

RT-PCR Amplification of p65 cDNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
In RT-PCR assays, the One-Step RT-PCR System (the Superscript III with Platinum Taq Invitrogen, Carlsbad, CA, USA) was used. To amplify the p65 cDNA, primers specific to p65 gene and three-step cycling, were used: 1) pre-denaturation at 55°C for 30 minutes; 2) 40 cycles of PCR amplification for 15-second, denaturing at 94°C, 30-second annealing at 60°C and 1 minute extension at 68°C; and 3) final extension for 5 minutes at 68°C. PCR products, 0.8 kb and 0.2 kb DNA were studied by DNA gel electrophoresis in a 1.5 % agarose gel.
Darbinian N., Darbinyan A., Merabova N., Gomberg R., Chabriere E., Simm M., Selzer M.E, & Amini S. (2020). DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65. Journal of HIV and AIDS, 6(1), 175.
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2

RT-PCR Amplification of p65 cDNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
In RT-PCR assays, the One-Step RT-PCR System (the Superscript III with Platinum Taq Invitrogen, Carlsbad, CA, USA) was used. To amplify the p65 cDNA, primers specific to p65 gene and three-step cycling, were used: 1) pre-denaturation at 55°C for 30 minutes; 2) 40 cycles of PCR amplification for 15-second, denaturing at 94°C, 30-second annealing at 60°C and 1 minute extension at 68°C; and 3) final extension for 5 minutes at 68°C. PCR products, 0.8 kb and 0.2 kb DNA were studied by DNA gel electrophoresis in a 1.5 % agarose gel.
Darbinian N., Darbinyan A., Merabova N., Gomberg R., Chabriere E., Simm M., Selzer M.E, & Amini S. (2020). DING Protein Inhibits Transcription of HIV-1 Gene through Suppression of Phosphorylation of NF-κB p65. Journal of HIV and AIDS, 6(1), 175.
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