Anti cd28 10f3

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-CD28 (10F3) is a monoclonal antibody that binds to the CD28 receptor on T cells. CD28 is a co-stimulatory molecule that plays a crucial role in T cell activation and proliferation. The Anti-CD28 (10F3) can be used in various immunological applications to study T cell biology and function.

Automatically generated - may contain errors

Lab products found in correlation

Human cd8 t cell enrichment kit, by STEMCELL (2 mentions) Anti cd3 okt3, by Thermo Fisher Scientific (2 mentions) Ficoll paque plus, by GE Healthcare (2 mentions) Ef450, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD28 (306 citations)

2 protocols using anti cd28 10f3

Isolation and Activation of CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors using Ficoll-Paque Plus (GE Healthcare). CD8⁺ T cells were isolated from PBMCs by magnetic bead purification using a human CD8⁺ T cell enrichment kit (STEMCELL Technologies). The purity (> 95%) was evaluated by flow cytometry using an eF450-labelled antibody against CD8 (48–0087–42, Thermo Fisher). CD8⁺ T cells were cultured in complete Roswell Park Memorial Institute (RPMI)-1640 medium. Then, CD8⁺ T cells (1 × 10⁵) were activated by stimulation with plate-bound anti-CD3 (OKT3, Thermo Fisher) at 2.5 µg/mL and anti-CD28 (10F3, Thermo Fisher) at 2 µg/mL in vitro.

Zhou C., Zhang Y., Yan R., Huang L., Mellor A.L., Yang Y., Chen X., Wei W., Wu X., Yu L., Liang L., Zhang D., Wu S, & Wang W. (2020). Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment. Cell Death and Differentiation, 28(2), 715-729.

+ Open protocol

+ Expand

Isolation and Activation of CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from HLA-A24 healthy donors using Ficoll-Paque Plus (GE Healthcare, Chicago, IL, USA). CD8⁺ T cells were isolated from the PBMCs by magnetic bead purification using a Human CD8⁺ T Cell Enrichment Kit (STEMCELL Technologies, Vancouver, BC, Canada). HLA-A24 phenotype and purity (>90%) were checked by flow cytometry using anti-HLA-A24 (human) monoclonal antibody (mAb)-phycoerythrin (PE) (K0208-5; MBL International) and eF450-labeled antibodies against CD8 (48-0087-42; Thermo Fisher Scientific, Waltham, MA, USA). CD8⁺ T cells were grown in complete RPMI-1640 medium plus interleukin (IL)-2 (10 IU/mL) and activated (1 × 10⁵ cells) by stimulation with plates coated with 2.5 μg/mL of anti-CD3 (OKT3; Thermo Fisher Scientific, Waltham, MA, USA) and 2 μg/mL of anti-CD28 (10F3; Thermo Fisher Scientific, Waltham, MA, USA) in vitro.

Zhou C., Wei W., Ma J., Yang Y., Liang L., Zhang Y., Wang Z., Chen X., Huang L., Wang W, & Wu S. (2021). Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels. Molecular Therapy, 29(4), 1512-1528.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!