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Quantikine tnf elisa kit

Manufactured by R&D Systems
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The Quantikine TNF ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure human Tumor Necrosis Factor (TNF) levels in cell culture supernates, serum, and plasma. The kit utilizes a monoclonal antibody specific for TNF immobilized on a microplate and a polyclonal antibody conjugated to horseradish peroxidase for detection.

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Lab products found in correlation

Phorbol myristate acetate (pma), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

Spelling variants of this product

Quantikine ELISA kit (1163 citations) Quantikine ELISA (398 citations) ELISAs (144 citations) Quantikine kits (80 citations) Quantikines (4 citations) Quantikine ELISA kits for TNF-α (3 citations) TNF Quantikine ELISA Kits (2 citations)

2 protocols using quantikine tnf elisa kit

1

Macrophage TNF-α Production Assay

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3–4 days post infection, macrophages were stimulated with 200 nm PMA (Invitrogen) or 100 ng/ml LPS (Sigma) in OPTIMEM supplemented with 1 mg/ml BSA for indicated times. ELISAs were performed with a Quantikine TNF ELISA kit according to the manufacturer’s instructions (R&D Systems, catalogue number MTA00B). Error bars represent standard deviations, and all two-tailed student t-tests represent a comparison with iRhom2WT data points.
Grieve A.G., Xu H., Künzel U., Bambrough P., Sieber B, & Freeman M. (2017). Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. eLife, 6, e23968.
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2

Quantifying TNF Protein Secretion

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Quantikine TNF ELISA kit (R&D Systems) was used according to manufacturer’s instructions for measuring TNF protein concentration (39 (link)). Cells were washed twice with PBS and resuspended to a density of 80(10)4 cell/mL in appropriate media before incubation with or without LPS. Supernatant of resulting cultures was collected when indicated and used for assay.
Millet P., Vachharajani V., McPhail L., Yoza B, & McCall C. (2016). GAPDH Binding to TNF-α mRNA Contributes to Post-Transcriptional Repression in Monocytes: A Novel Mechanism of Communication between Inflammation and Metabolism. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2541-2551.
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