Hydroxymethyl aminomethane hydrochloride tris hcl

Manufactured by Merck Group

Sourced in Germany

Hydroxymethyl aminomethane hydrochloride (Tris-HCl) is a chemical compound commonly used as a buffer in various laboratory applications. It is a white, crystalline solid that is soluble in water and has a pH-buffering capacity. The primary function of Tris-HCl is to maintain a specific pH range in chemical reactions and biological assays, enabling the optimal performance of enzymes and other biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Tris-HCl (658 citations) Tris(hydroxymethyl)aminomethane (144 citations) Tris(hydroxymethyl)aminomethane (Tris) (82 citations) Tris hydrochloride (63 citations) Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) (23 citations) Tris hydrochloride (Tris-HCl) (17 citations) Tris(hydroxymethyl)aminomethane hydrochloride (15 citations) Tris(hydroxymethyl)aminomethane-HCl (3 citations) Hydrochlorides (2 citations)

11 protocols using hydroxymethyl aminomethane hydrochloride tris hcl

Oxidative Stress Evaluation of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

2′,7′ Dichlorofluorescein diacetate (DCFH-DA), 3-(N-morpholino) propane sulfonic acid (MOPS), 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), thiobarbituric acid (TBA), glutathione (GSH), malondialdehyde (MDA), eosin, Coomassie brilliant blue, nigrosin, 2, 4-dinitrofluorobenzene (DNFB), dinitrophenylhydrazine (DNPH), sucrose, KCl, NaCl, dithiothreitol (DTT), Na₂HPO₄, MgCl₂, Rhodamine 123 (Rh 123), ethylenediaminetetraacetic acid (EDTA), were purchased from Sigma Chemical Co. (St. Louis, MO, USA). SWCNT and MWCNT were prepared from US Research nanomaterials Inc. (Houston, TX 77084, USA; Please refer to the Supplementary Material for more information). Trichloroacetic acid (TCA) and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were purchased from Merck (Darmstadt, Germany). The salts for preparing buffer solutions were of the analytical grade and obtained from Merck (Darmstadt, Germany).

Farshad O., Heidari R., Zamiri M.J., Retana-Márquez S., Khalili M., Ebrahimi M., Jamshidzadeh A, & Ommati M.M. (2020). Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice. Frontiers in Veterinary Science, 7, 591558.

+ Open protocol

+ Expand

Oxidative Stress Biomarker Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

4,2-Hydroxyethyl,1-piperazine ethane sulfonic acid (HEPES), thiobarbituric acid (TBA), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (trolox), sucrose, fatty acid-free bovine serum albumin (BSA) fraction V, Coomassie brilliant blue, dithiobis-2-nitrobenzoic acid (DTNB), malondialdehyde (MDA), glutathione (GSH), 2′,7′-Dichlorofluorescein diacetate (DCFH-DA), potassium chloride (KCl), sodium phosphate dibasic (Na₂HPO₄), sodium phosphate monobasic (NaH₂PO₄), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Ammonium chloride, Trichloroacetic acid (TCA), and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were purchased from Merck (Darmstadt, Germany). Citicoline was purchased from Alborz Darou^® (Tehran, Iran). Commercial kits were used for the assessment of plasma biomarkers (Pars Azmoon^®, Tehran, Iran).

Farshad O., Keshavarz P., Heidari R., Farahmandnejad M., Azhdari S, & Jamshidzadeh A. (2020). The Potential Neuroprotective Role of Citicoline in Hepatic Encephalopathy. Journal of Experimental Pharmacology, 12, 517-527.

+ Open protocol

+ Expand

Neuroprotective Agents in Parkinson's Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

Desipramine‐HCl and regular human insulin were purchased from Exir Co. (Tehran, Iran). 6‐hydroxydopamine hydrochloride (6‐OHDA), apomorphine, paraformaldehyde (PFA), hydrogen peroxide, trichloroacetic acids (TCA), hydroxymethyl aminomethane hydrochloride (Tris‐HCl), and Rhodamine 123 were purchased from Merck Co. (Darmstadt, Germany). Horseradish peroxidase (HRP), 3, 3′‐Diamino benzidine tetrahydrochloride (DAB), mannitol, sucrose, 4,2‐hydroxyethyl,1‐piperazineethanesulfonic acid (HEPES), 3‐[4,5dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), fatty acid‐free bovine serum albumin (BSA), dithiobis‐2‐nitrobenzoic acid (DTNB), glutathione (GSH), 2′,7′‐dichlorofluorescein diacetate (DCFH‐DA), potassium chloride (KCl), ethylene di‐amine tetra acetic acid (EDTA), ethylene glycol‐bis (2‐aminoethyl ether)‐N,N,N′,N′‐tetra acetic acid (EGTA) and Bradford reagent were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Rabbit polyclonal anti‐TH antibody was purchased from Abcam Co. (USA). SYBR Green Real‐Time PCR Master Mix was purchased from Ampliqon Co (Denmark).

Iravanpour F., Dargahi L., Rezaei M., Haghani M., Heidari R., Valian N, & Ahmadiani A. (2021). Intranasal insulin improves mitochondrial function and attenuates motor deficits in a rat 6‐OHDA model of Parkinson's disease. CNS Neuroscience & Therapeutics, 27(3), 308-319.

+ Open protocol

+ Expand

Carnosine Protective Effects Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carnosine was purchased from Sigma (St. Louis, MO, USA). 4,2-Hydroxyethyl,1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino) propane sulfonic acid (MOPS), Dimethyl sulfoxide (DMSO), D-mannitol, bovine serum albumin (BSA), thiobarbituric acid (TBA), 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), Coomassie brilliant blue, Rhodamine 123 (Rh 123), Ethylene glycol-bis (2-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), Sodium succinate, Hydroxymethyl aminomethane hydrochloride (Tris-HCl), and ethylenediaminetetraacetic acid (EDTA) were purchased from Merck (Darmstadt, Germany). All salts for preparing buffer solutions (analytical grade) were purchased from Merck (Darmstadt, Germany).

Ghanbarinejad V., Ahmadi A., Niknahad H., Ommati M.M, & Heidari R. (2019). Carnosine Mitigates Manganese Mitotoxicity in an In Vitro Model of Isolated Brain Mitochondria. Advanced Pharmaceutical Bulletin, 9(2), 294-301.

+ Open protocol

+ Expand

Oxidative Stress Assays Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Meta-phosphoric acid, 2-mercaptoethanol, 2,7-dichlorofluorescein diacetate (DCF-DA), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), ethylenediamine-tetraacetic acid (EDTA), GSH, ferric chloride hexahydrate (FeCl₃.6H₂O), and 5,5-dit hiobis-2-nitrobenzoic acid (DTNB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium and potassium phosphates, saponin, trichloroacetic acid, sodium hydrogen phosphate dibasic, thiobarbituric acid, sodium chloride, sodium citrate, sodium hydrogen phosphate monobasic, n-butanol, methanol, and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were obtained from Merck (Darmstadt, Germany). G6PD activity was determined using a commercial kit (Randox Laboratories, Antrim, United Kingdom). Commercially available Giemsa was purchased from ATR-MED (Tehran, Iran).

Niknahad H., Mehrabani P.S., Arjmand A., Alidaee S., Mazloomi S., Ahmadi P., Abdoli N., Saeed M., Rezaei M., Ommati M.M, & Heidari R. (2023). Cirrhosis-induced oxidative stress in erythrocytes: The therapeutic potential of taurine. Clinical and Experimental Hepatology, 9(1), 79-93.

+ Open protocol

+ Expand

Oxidative Stress Evaluation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

4,2-Hydroxyethyl, 1-piperazineethanesulfonic acid (HEPES), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carbo 2-carboxylic acid (Trolox), 3-(N-morpholino) propane sulfonic acid (MOPS), dimethyl sulfoxide (DMSO), D-mannitol, fatty acid-free bovine serum albumin (BSA) fraction V, Thiobarbituric acid (TBA), 3-[4 (link), 5 (link) dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT), Dithiobis-2-nitrobenzoic acid (DTNB), Glutathione (GSH), 2’, 7’-Dichlorofluorescein diacetate (DCFH-DA), Taurine, Malondialdehyde (MDA), Sucrose, KCl, Na₂HPO₄, MgCl₂, Rhodamine 123 (Rh-123), Coomassie brilliant blue, Ethylene glycol-bis (2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA), Sodium succinate, and Ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trichloroacetic acids (TCA), ammonium chloride, and hydroxymethyl amino methane hydrochloride (Tris-HCl) were purchased from Merck (Dardamstd, Germany). All salts for preparing buffer solutions were of analytical grade and prepared from Merck (Dardamstd, Germany).

Niknahad H., Jamshidzadeh A., Heidari R., Zarei M, & Ommati M.M. (2017). Ammonia-induced mitochondrial dysfunction and energy metabolism disturbances in isolated brain and liver mitochondria, and the effect of taurine administration: relevance to hepatic encephalopathy treatment. Clinical and Experimental Hepatology, 3(3), 141-151.

+ Open protocol

+ Expand

Antioxidant and Oxidative Stress Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

4,2-Hydroxyethyl,1-piperazine ethane sulfonic acid (HEPES), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), phenol, thiobarbituric acid (TBA), reduced GSH, rhodamine 123, 2´,7´-dichlorofluorescein diacetate (DCFH-DA), malondialdehyde (MDA), potassium chloride (KCl), sodium phosphate dibasic (Na₂HPO₄), ethylenediaminetetraacetic acid (EDTA), oxidized glutathione (GSSG), Coomassie brilliant blue, methanol HPLC grade, ethylene glycol-bis (2-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA), mannitol, acetonitrile HPLC grade, sucrose, and NAC were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Acetyl-para-aminophenol (APAP; acetaminophen), trichloroacetic acid (TCA), calcium chloride, hydroxymethyl aminomethane hydrochloride (Tris-HCl), and ammonium chloride were purchased from Merck (Darmstadt, Germany). All salts for preparing buffer solutions were of analytical grade and prepared from Merck (Darmstadt, Germany).

Mohammadi H., Sayad A., Mohammadi M., Niknahad H, & Heidari R. (2020). N-acetyl cysteine treatment preserves mitochondrial indices of functionality in the brain of hyperammonemic mice. Clinical and Experimental Hepatology, 6(2), 106-115.

+ Open protocol

+ Expand

Antioxidant Activity Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fatty acid-free bovine serum albumin (BSA) fraction V, dithiobis-2-nitrobenzoic acid (DTNB), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), 4,2hydroxyethyl,1-piperazine ethane sulfonic acid (HEPES), thiobarbituric acid (TBA), 2′,7′dichlorofluorescein diacetate (DCFH-DA), taurine (TAU), malondialdehyde (MDA), glutathione (GSH), sodium phosphate dibasic (Na₂HPO₄), sucrose, potassium chloride (KCl), Coomassie brilliant blue, dithiothreitol, ethylene glycol-bis (2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Hydroxymethyl aminomethane hydrochloride (Tris-HCl), and trichloroacetic acid (TCA) were obtained from Merck (Darmstadt, Germany). All salts (analytical grade) for preparing buffer solutions were obtained from Merck (Darmstadt, Germany).

Heidari R., Jamshidzadeh A., Ghanbarinejad V., Ommati M.M, & Niknahad H. (2018). Taurine supplementation abates cirrhosis-associated locomotor dysfunction. Clinical and Experimental Hepatology, 4(2), 72-82.

+ Open protocol

+ Expand

Evaluation of Oxidative Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

2′,7′-Dichlorofluorescein diacetate (DCFH-DA), 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 4,2-Hydroxyethyl,1-piperazineethanesulfonic acid (HEPES), oxidized glutathione, dinitro fluoro benzene, bovine serum albumin, lithium chloride (Li⁺), 3-(N-morpholino) propane sulfonic acid (MOPS), ethylene glycol-bis (2-aminoethyl ether)-N, N, N′, N′-tetraacetic acid (EGTA), dimethyl sulfoxide, iodo-acetic acid, nigrosine, glutathione (GSH), malondialdehyde, potassium chloride, eosine, thiobarbituric acid, coomassie brilliant blue, sucrose, sodium chloride, dithiothreitol, rhodamine 123, and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trichloroacetic acid (TCA) and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were purchased from Merck (Darmstadt, Germany).

Ommati M.M., Arabnezhad M.R., Farshad O., Jamshidzadeh A., Niknahad H., Retana-Marquez S., Jia Z., Nateghahmadi M.H., Mousavi K., Arazi A., Azmoon M.R., Azarpira N, & Heidari R. (2021). The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice. Frontiers in Veterinary Science, 8, 603262.

+ Open protocol

+ Expand

Oxidative Stress Biomarker Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium succinate, fatty acid-free bovine serum albumin (BSA) fraction V, thiobarbituric acid (TBA), 4,2-hydroxyethyl,1-piperazineethanesulfonic acid (HEPES), manganese chloride, dimethyl sulfoxide (DMSO), ammonium chloride, D-mannitol, 3-(N-morpholino)-propane sulfonic acid (MOPS), n-butanol, 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 2’,7’dichlorofluorescein (DCF), sucrose, rhodamine 123 (Rh 123), Coomassie brilliant blue, and ethylene glycol-bis-(2-amino-ethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Hydroxymethyl aminomethane hydrochloride (Tris-HCl) and trichloroacetic acid (TCA) were purchased from Merck (Darmstadt, Germany). All salts for preparing buffers were of the analytical grade and obtained from Merck (Darmstadt, Germany).

Heidari R., Jamshidzadeh A., Ommati M.M., Rashidi E., Khodaei F., Sadeghi A., Hosseini A, & Niknahad H. (2019). Ammonia-induced mitochondrial impairment is intensified by manganese co-exposure: relevance to the management of subclinical hepatic encephalopathy and cirrhosis-associated brain injury. Clinical and Experimental Hepatology, 5(2), 109-117.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!