The sections were deparaffined in Histoclear for 15 min (2 times), rehydrated in ethanol diminishing gradient (100%, 96%, 75% and water: 1 min each) and unmasked in Tris-EDTA buffer (10 mM Tris-Base, 1 mM EDTA, pH 9) during 20 min at 95 °C. The sections were incubated with blocking buffer (1% bovin serum albumin in PBS) for 1 h at room temperature and then with anti-phospho-H2AX (dilution 1/500, #9718, Cell Signaling, Danvers, MA, USA) overnight at 4 °C. After several washes with PBS, the sections were incubated with the corresponding secondary antibody coupled with peroxydase (dilution 1/500, #111-065-003, Jackson Immunoresearch, Ely, Cambridgeshire, United Kingdom) for 2 h at room temperature. Revelation was performed using 3,3′-Diaminobenzidine (#SK-4800, NovaRED, Vector Laboratories, Burlingame, CA, USA). The sections were counterstained with Mayer hematoxilin (Diapath, Martinengo (BG) Italy) for 10 sec, rinsed under running water, dehydrated and mounted in Eukitt mounting solution. The microscopic images were acquired using the Scanner Zeiss Axioscan Z1 (Zeiss, Jena, Germany) and analysed using ZEN 2 software.
Peroxydase
Manufactured by Jackson ImmunoResearch
Sourced in United Kingdom
Peroxydase is a laboratory reagent used in various biochemical and immunological assays. It is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide. Peroxydase can be used as a detection system in applications such as enzyme-linked immunosorbent assays (ELISA) and Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peroxydase
1
Immunohistochemical Analysis of DNA Damage
2
Immunohistochemical Staining of Colonic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The colonic sections were deparaffined in HISTO-CLEAR (Electron Microscopy Sciences, 64110-04) for 15 min (2 times), rehydrated in ethanol diminishing gradient (100%, 96%, 75% and water: 1 min each) and unmasked in Tris-EDTA buffer (10 mM Tris-Base, 1 mM EDTA, pH 9) during 20 min at 95°C. The sections were incubated with blocking buffer (1% bovine serum albumin [Euromedex, 04-100812-E] in PBS) for 1 h at room temperature and then with anti-phopho-H2AX (dilution 1:500; Cell Signaling Technology, 9718), or anti-MKI67/Ki67 (dilution 1:500; Cell Signaling Technology, 12202S) overnight at 4°C. After several washes with PBS, the sections were incubated with the corresponding secondary antibody coupled with peroxydase (dilution 1:500; Jackson Immunoresearch, 111-065-003) for 2 h at room temperature. Revelation was performed using 3,3'-diaminobenzidine (NovaRED; Vector Laboratories, SK-4800). The sections were counterstained with Mayer's hematoxylin solution (Sigma-Aldrich, MHS32) for 10 sec, rinsed under running water, dehydrated and mounted using a 50:50 PBS-glycerol solution. The microscopic images were acquired using the Scanner Zeiss Axioscan Z1 (Zeiss) and analyzed using ZEN 2 software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!