The Morris water maze (MWM) system (Noldus, Netherlands); inverted fluorescence biomicroscope (Zeiss, Germany); E0970 Microtome Cryostat (Beyotime Biotech, Beijing, China); enhanced chemiluminescence (ECL) detection system (Bio-Rad, Hercules, CA, United States); Multiskan FC microplate reader (Thermo Scientific, United States); western blot Electrophoresis Instrument and Trans-Blot instrument (Bio-Rad, United States) were used in this study.
Trans blot instrument
Manufactured by Bio-Rad
Sourced in United States
The Trans-Blot instrument is a laboratory equipment used for the transfer of proteins or nucleic acids from a gel to a membrane. It is a critical component in various analytical techniques, such as Western blotting and Northern blotting, allowing for the efficient and reproducible transfer of biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Western blot electrophoresis instrument, by Bio-Rad (1 mentions)
Multiskan fc microplate reader, by Thermo Fisher Scientific (1 mentions)
Ecl detection system, by Bio-Rad (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
Sodium orthovandate, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Roche (1 mentions)
3 protocols using trans blot instrument
1
Detailed Neurobehavioral Assays and Analysis
2
Western Blot Analysis of Survivin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549 cells were harvested and lysed in RIPA buffer, and the concentration of the obtained proteins was quantified using the BCA protein assay kit. Then the proteins were electrophoresed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes using a Trans-blot instrument (Bio-Rad, USA). The membranes were incubated with a survivin rabbit monoclonal antibody (1 : 1000), followed by incubating with HRP conjugated goat anti-rabbit IgG (H + L) as a secondary antibody (1 : 2500). An anti-β-actin antibody was used as a control. The immunoblots were visualized with chemiluminescence and analyzed using a ChemiDoc XRS+ imaging system (Bio-Rad, USA).
3
Quantifying CD147 Expression in Statin-Treated THP-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
THP-1 cells were preincubated with statins or controls and differentiated with PMA for 24 h. Cells were then lysed in 1 ml RIPA buffer supplemented with sodium orthovandate (Sigma) and a complete protease inhibitor cocktail (Roche, Indianapolis, IN) for 45 min with intermittent shaking on ice. The proteins were separated on a 10% SDS-PAGE gel and transferred to a PVDF membrane using a Transblot instrument (Biorad, Hercules, CA) at 20 V for 1 h. The immunoblot was blocked for 2 h in 5% lipid free milk and incubated with an anti-CD147 antibody (Immunotools) for 1 h, followed by a 30 min wash. The primary antibody was conjugated to goat anti-mouse secondary antibody-horseradish peroxidase (HRP; Becton Dickinson) and incubated for 1 h. Finally, the blot was developed using an ECL development system (Amersham, Buckinghamshire, UK) or an enhanced ECL system (Pierce, Rockford, IL). Images were analyzed using ImageJ software (NIH, Bethesda, MD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!