The following antibodies were used, adding 1 µL of each antibody to our sample per our previously published work6 (link): EpCAM (Biolegend), CD104 (Thermo Fisher), c-Met (Thermo Fisher), CD44v6 (Thermo Fisher), Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM, CD104) and IgGK1 (c-Met, CD44v6) alongside the Miltenyi REA Isotype control (to match Tspan8). Samples were run at a flow rate of ɸ = 2.5 mL/h.
Cd104
Manufactured by Thermo Fisher Scientific
The CD104 is a laboratory instrument designed for the analysis and detection of specific cell surface markers. It utilizes flow cytometry technology to quantify the expression of the CD104 antigen on cells. The CD104 instrument provides accurate and reliable data to support researchers in their investigations of cell biology and immunology.
Automatically generated - may contain errors
Lab products found in correlation
C met, by Thermo Fisher Scientific (2 mentions)
Cd44v6, by Thermo Fisher Scientific (2 mentions)
Tspan8, by Miltenyi Biotec (2 mentions)
Iggk2b, by BioLegend (2 mentions)
Cd104, by Miltenyi Biotec (2 mentions)
Iggk1, by Miltenyi Biotec (2 mentions)
C met, by Miltenyi Biotec (2 mentions)
Cd44v6, by Miltenyi Biotec (2 mentions)
Rea isotype control, by Miltenyi Biotec (2 mentions)
Epcam, by Miltenyi Biotec (2 mentions)
Epcam, by BioLegend (2 mentions)
Snai1, by Cell Signaling Technology (1 mentions)
Cd44 antibody, by Proteintech (1 mentions)
Cd44 pe, by Thermo Fisher Scientific (1 mentions)
Cd105, by BD (1 mentions)
Cd146, by BD (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
3 protocols using cd104
1
Multimarker Flow Cytometry Profiling
2
Expression of Stemness and EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Vimentin (Cat#5741), SNAI1 (Cat #3879), ZEB1 (Cat #3396), SOX2 (Cat #3579), E-Cadherin (Cat #14472), TWIST1(Cat #46702), Ki67(Cat #9129) were purchased from Cell Signal Technology. Antibody against EBV LMP1 was purchased from Abcam (Cat #ab78113). Antibody against EBV LMP2A was purchased from Santa Cruz Biotechnology (Cat #sc-101314). CD44 PE (Cat #12-0441-81), CD104 (Cat #PA5-79541), CD104 eFluor 660 (Cat #50-1049-82) antibodies were purchased from ThermoFisher Scientific. CD44 antibody was purchased from Proteintech (Cat #15675-1-AP). Antibodies against CD90 (Cat #555596), CD146 (Cat #550315), CD105 (Cat #560839), CD166 Antibody (Cat #560903) were purchased from BD Bioscience.
3
Characterization of Circulating Tumor Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
the following antibodies were used, adding 1 μL of each antibody to our sample per our previously published work [6 ]: EpCAM (Biolegend), CD104 (Thermo Fisher), c-Met (Thermo Fisher), CD44v6 (Thermo Fisher), Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM, CD104) and IgGK1 (c-Met, CD44v6) alongside the Miltenyi REA Isotype control (to match Tspan8). Samples were run at a flow rate of = 2.5 mL/hr.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!