Lipid hydroperoxide, 8-hydroxy-2'-deoxyguanosine (8-OHdG), 8-nitroguanine, PARP1 activity and adenosine triphosphate (ATP) assays were performed with whole kidneys or cells using a lipid hydroperoxide assay kit (Cayman, Ann Arbor, MI, USA), a DNA/RNA oxidative damage ELISA kit (Cayman), a 8-nitroguanine DNA/RNA damage ELISA kit (Cell Biolabs, San Diego, CA, USA), a universal PARP colorimetric assay kit (Trevigen, Gaithersburg, MD, USA), and an ATP fluorometric assay kit (BioVision, Mountain View, CA, USA) according to the respective manufacturer's instructions [25 (link)].
8 nitroguanine dna rna damage elisa kit
Manufactured by Cell Biolabs
Sourced in United States
The 8-nitroguanine DNA/RNA Damage ELISA Kit is a quantitative assay designed to measure the levels of 8-nitroguanine, a marker of oxidative and nitrative DNA/RNA damage, in biological samples. The kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA) format to detect and quantify 8-nitroguanine concentrations.
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2 protocols using 8 nitroguanine dna rna damage elisa kit
1
Kidney Oxidative Stress Assays
2
Oxidative Stress Markers in Kidney
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PARP1 activity, 8-nitroguanine, and 8-hydroxy-2′-deoxyguanosine assays were performed in whole kidneys using a universal PARP colorimetric assay kit (Trevigen, Gaithersburg, MD, USA), a 8-nitroguanine DNA/RNA damage ELISA kit (Cell Biolabs, San Diego, CA, USA) and a DNA/RNA oxidative damage ELISA kit (Cayman, Ann Arbor, MI, USA), respectively, according to the manufacturer's instructions [18 (link)19 (link)22 (link)23 (link)24 (link)25 (link)].
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