0.2 μm filter

Manufactured by Thermo Fisher Scientific

The 0.2 μm filter is a laboratory equipment designed for filtration purposes. It is capable of removing particles or microorganisms from liquids or gases that are 0.2 micrometers or larger in size. The filter provides a physical barrier to separate components based on their size.

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Lab products found in correlation

Ascorbic acid, by Thermo Fisher Scientific (2 mentions) Magnesium chloride mgcl2, by Avantor (2 mentions) Tetrabutylammonium chloride tbacl 1 2 dichloroethane dce chlorotrimethylsilane, by Merck Group (2 mentions) Dopamine hydrochloride, by Merck Group (2 mentions) N octyl β d glucopyranoside, by Merck Group (1 mentions) Pe labeled streptavidin, by Thermo Fisher Scientific (1 mentions) Cnbr activated sepharose 4b, by GE Healthcare (1 mentions) Polyvinyl acetate, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Thermo Fisher Scientific (1 mentions) Cocaine hydrochloride, by Thermo Fisher Scientific (1 mentions)

5 protocols using 0.2 μm filter

HLA Class II Monomer and Tetramer Generation

Cited 3 times

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Myc-tagged DRA1/DRB1*0301 HLA class II and untagged DRA1/DRB1*0301, DRA1/DRB1*1101 and DRA1/DRB1*1501 monomer reagents were generated essentially as previously described^{29 (link),35 (link)}. In brief, transfected S2 cells were expanded to a 2 L volume in spinner flasks (Bellco, Vineland NJ) and induced for 5 days with 1 mM copper sulfate, adding 2 μg mL⁻¹ biotin to ensure efficient protein biotinlyation. Supernatants were separated from intact cells by centrifugation (11,000g), separated from debris using a 0.2 μm filter (ThermoFisher, Waltham, MA) and then affinity purified using L243 coupled with CNBr-Activated Sepharose 4B (GE Healthcare, Pittsburgh, PA). Class II protein was eluted at pH 11.5, equilibrated using pH 4.0 Tris buffer and exchanged into a pH 6.0 storage buffer (0.2 M sodium phosphate). Class II monomers were loaded with individual peptides by incubating for 72 h at 37°°C in the presence of 2.5 mg mL⁻¹n-octyl-β-d-glucopyranoside (Sigma, St Louis, MO). Tetramers were formed by individually incubating class II molecules with metal labeled streptavidin or PE-labeled streptavidin (Thermo Fisher Scientific) for 6–18 h at room temperature at a molar ratio of 8:1. Metal labeled streptavidin were produced as described earlier^{36 (link),37 (link)}.

DeGottardi Q., Gates T.J., Yang J., James E.A., Malhotra U., Chow I.T., Simoni Y., Fehlings M., Newell E.W., DeBerg H.A, & Kwok W.W. (2020). Ontogeny of different subsets of yellow fever virus-specific circulatory CXCR5+ CD4+ T cells after yellow fever vaccination. Scientific Reports, 10, 15686.

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Electrochemical Characterization of Dopamine

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Potassium tetrakis(pentafluorophenyl)borate (TFAB) was obtained from Boulder Scientific Company (Mead, CO). Dopamine hydrochloride, dibenzo-18-crown-6 (DB18C6), tetradodecylammonium (TDDA) chloride, tetrabutylammonium chloride (TBACl), 1,2-dichloroethane (DCE), chlorotrimethylsilane were purchased from Sigma-Aldrich (St. Louis, MO). The TFAB salt of TDDA (TDDATFAB) was prepared by metathesis, as described elsewhere(49). Magnesium chloride (MgCl₂) was from Amresco (Solon, OH). Ascorbic acid was from Fisher Scientific (Pittsburgh, PA). All reagents were used as received, and solutions were prepared using 18.3 MΩ cm deionized water (ELGA, Woodridge, IL). The prepared solutions were passed through a 0.2 μm filter (Thermo Scientific, Waltham, MA) before use.

Colombo M.L., McNeil S., Iwai N., Chang A, & Shen M. (2015). Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry. Journal of the Electrochemical Society, 163(4), H3072-H3076.

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Electrochemical Characterization of Dopamine

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Double Emulsion Microsphere Synthesis

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A double emulsion technique was used to synthesize microspheres with poly (ε-caprolactone) (PCL) and poly (lactic-co-glycolic acid) (PLGA, 85:15) (both Sigma-Aldrich, St. Louis, MO) as described previously.^{21 (link)} Briefly, a 100-μL quantity of payloads (Protoporphyrin-IX, Fluorescein-conjugated immunoglobulin, Albumin, TGF-β1, or BMP4) were pipetted into 1 mL of 5% PCL or PLGA in ethyl acetate (Sigma-Aldrich, St. Louis, MO) and immediately sonicated (QSonica, Newtown, CT) for 1 min. A second emulsion composed of 1% polyvinyl acetate (PVA) (Sigma-Aldrich, St. Louis, MO) and 7% ethyl acetate was then added and vortexed for 15 s. This emulsion was then added to a 1% PVA solution with continuous stirring for 3 h at room temperature, filtered through a 0.2 μm filter (ThermoFisher Scientific, Waltham, MA), collected by centrifugation, freeze-dried (Labconco, Kansas City, MO), and stored at −20°C till further use.

Rahman S.U., Ponnusamy S., Nagrath M, & Arany P.R. (2022). Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues. Journal of Tissue Engineering, 13, 20417314211073934.

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Cocaine and 5-HT1B Receptor Modulation

Cited 1 time

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Cocaine hydrochloride (provided by the National Institute on Drug Abuse) was dissolved in 0.9% physiological saline and sterile filtered through a 0.2μm filter (ThermoScientific). Cocaine was diluted to a dose of 1 mg/kg delivered in a volume of 0.1 ml over a period of 4.3 s via a 10ml syringe nested in a motorized syringe pump (Razel Scientific Instruments). The dose of 1 mg/kg i.v. cocaine was chosen based upon the results of previous runway work from our laboratory (Raven et al 2000 (link); Ettenberg 2004 (link); Ettenberg and Bernardi 2006 (link); Wenzel et al 2011 (link); 2014 (link)).
The 5-HT_1B agonist CP 94,253 dihydrochloride (Sigma-Aldrich) was prepared in a vehicle solution of aCSF (l-Ascorbic Acid 0.35g/L, NaCl 8.47g/L, KCl .20g/L, MgCl₂ .20g/L, CaCl₂ .18g/L, NaH₂PO₄ .276g/L, Na₂HPO₄ .5362g/L) for intracranial infusion at the concentrations 0.25, 0.5, or 1.0μg/0.5μl. CP 94,253 was selected as it shows the greatest affinity for 5-HT_1B over other receptors in the 5-HT₁ family (Koe et al 1992 ). Utilized doses were determined from prior studies reporting behavioral effects with intracerebral administration (De Almeida et al 2006 (link); Veiga and Miczek 2007 ). The selective 5-HT_1B antagonist NAS-181 (Stenfors et al 2000 (link); De Groote et al 2002 (link); 2003 (link)) was prepared in the same vehicle as CP 94,253 and infused at doses of 0.1μg or 1.0μg per 0.5μl/side.

Klein A.K., Brito M.A., Akhavan S., Flanagan D.R., Le N., Ohana T., Patil A.S., Purvis E.M., Provenzano C., Wei A., Zhou L, & Ettenberg A. (2016). Attenuation of the anxiogenic effects of cocaine by 5-HT1B autoreceptor stimulation in the Bed Nucleus of the Stria Terminalis of rats. Psychopharmacology, 234(3), 485-495.

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