Tris hydroxymethyl methyl amino methane

Steviol (99% of purity, HPLC) was purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). 5-Fluorouracil (5-FU), dimethyl sulfoxide (DMSO), Na₂CO₃, NaHCO₃, NaCl, KCl, Na₂HPO₄·12H₂O, NaH₂PO₄·2H₂O, EDTA disodium, dodecyl sodium sulfate (SDS), glycine, bromoxylenol blue, ammonium persulphate, tris (hydroxymethyl) methyl amino methane, ponceau, N,N,N, N-tetramethylethylenediamine (TEMED), xylene brilliant cyanin G (BS, G250), and phenylmethylsulfonyl fluoride (PMSF) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Trypsin-EDTA solution, propidium iodide (PI), triton X-100, endonuclease (RNase A), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), penicillin-streptomycin solution (100X), bovine albumin (BSA), BeyoECL Plus, polyvinylidene fluoride, RIPA lysis buffer, and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyasix iodide (JC-1) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). DMEM medium, fetal bovine serum were purchased from Gibco Life Technologies Corporation (Carlsbad, CA, USA). Primary antibodies against p21, p53, cyclin D1, Bax, Bcl-2, caspase 3, β-actin, and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signal Technologies Inc. (Beverly, MA, USA). All other reagents were of analytical grade and used as received unless otherwise stated.

Rat RSC96 SC line and rat vascular vein EC line were purchased from Bangchen Co., Ltd, Shanghai. Sprague–Dawley (SD, 1 day) suckling mice of Specific Pathogen Free (SPF) were provided by the Experimental Animal Center of Nantong University. Chitosan was obtained from Xingcheng Biological Products Factory (Jiangsu, China). AS powder was purchased from Ningxia Oasis Grass Industry Co., Ltd. Penicillin–streptomycin, DA, 4′,6-diamidino-2-phenylindole (DAPI), cytarabine and poly-D-lysine were all obtained from Sigma-Aldrich in the USA. Collagenase, trypsin, fetal bovine serum (FBS), 1640 medium, neurobasal medium, Dulbecco’s modified Eagle’s medium (DMEM) and high-glucose basal medium were all purchased from Gibco, USA. Phosphate-buffered saline (PBS, pH = 7) was purchased from HyClone. Paraformaldehyde came from the National Pharmaceutical Group Chemical Reagent Co., Ltd, China. Tris (hydroxymethyl) methyl aminomethane was bought from Sinopharm Chemical Reagent Co., Ltd, China. Immunofluorescence blocking solution, primary antibody diluent and secondary antibody diluent were purchased from Beyotime Biotechnology Co., Ltd, Shanghai. Rabbit monoclonal [4B3] to S100, goat anti-rabbit IgG H&L (Cy3 ®) pre-adsorbed, mouse monoclonal [2G10] to beta III tubulin-neuronal marker and goat anti-mouse IgG H&L (Alexa Fluor 488) were provided by Abcam.