The TGFβ1 concentration in the supernatant was measured with a Quantikine ELISA Human TGFβ1 kit (R&D Systems). A2780 cells were first subjected to siRNA-mediated gene silencing treatment for 48 h. A total of 6×105 cells were suspended in 1.5 ml of culture medium, added to a single well of a six‐well ultralow-attachment plate, and then cultured for 72 h. The culture medium was not changed during the culture period. Then, the culture medium was collected in a 1.5 ml conical tube and centrifuged at 3,000 rpm for 15 minutes. The concentration of TGFβ1 in the culture medium was determined using a human TGFβ1 Quantikine ELISA kit (R&D Systems). HCl and NaOH/HEPES were used to convert latent TGFβ1 into the activated form.
Quantikine elisa human tgfβ1 kit
Manufactured by R&D Systems
Sourced in United States
The Quantikine ELISA Human TGFβ1 kit is a quantitative sandwich enzyme immunoassay designed to measure human transforming growth factor beta 1 (TGFβ1) levels in cell culture supernates, serum, and plasma. It utilizes a microplate pre-coated with a monoclonal antibody specific for TGFβ1.
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Lab products found in correlation
5 protocols using quantikine elisa human tgfβ1 kit
1
Quantitative Analysis of TGFβ1 Secretion
2
Quantitative Analysis of Cytosolic TGF-β1
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Cytosolic TGF-β1: TGF-β1 concentration was measured using the Quantikine ELISA Human TGF-β1 Kit from R&D Systems (SB100B) as described by the manufacturer.
3
Quantification of TGF-β1 in Lung Tissue
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TGF-β1 in LA tissue lysates was measured with the Quantikine ELISA Human TGFβ1 kit (R&D Systems, Minneapolis, Minnesota). For details, see the Supplemental Appendix .
4
Quantification of TGF-β1 in Mouse Tissues
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For ELISA analysis, tissue was collected after perfusion with phosphate buffer solution and flash frozen in cold isopropyl alcohol. Mouse serum is collected by clotted blood without any anticoagulant for 30 min followed by centrifugation at 1500 g for 10 min at 4°C. Serum is collected from the supernatant and frozen at −80C. The tissue was sectioned with a cryostat to punch 2mm punches of tissue. Tissue was placed in RIPA buffer then homogenized using sonication at 30% amplitude, for 3 second pulses with 2 second pauses. BCA method was used to determine total protein concentration in the samples and Quantikine ELISA Human TGF-β1 kit (R&D Systems, Minneapolis, MN) was used to analyze TGF-β1 ligand levels following the instruction from the manufacturer.
5
Neurochemical Biomarker Assessment
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Plasma levels of 3-methoxy-4-hydroxyphenylglycol (MHPG) and homovanillic acid (HVA) were measured by high performance liquid chromatography. Brain-derived neurotrophic factor (BDNF) was measured using the Quantikine ELISA human BDNF kit (R&D Systems, Inc., Minneapolis, MN, USA), with a minimum detectable concentration of 20 pg/mL. TGF-β1 was assessed using the Quantikine ELISA human TGF-β1 kit (R&D Systems, Inc., Minneapolis, MN, USA), with a minimum detectable concentration of 0.50 pg/mL.
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