The largest database of trusted experimental protocols

96 well white flat bottom opaque tissue culture plate

Manufactured by BD

The 96-well white flat-bottom opaque tissue culture plate is a laboratory equipment designed for cell culture and bioassay applications. It features a white, flat-bottom, opaque well configuration with a standard 96-well format.

Automatically generated - may contain errors

2 protocols using 96 well white flat bottom opaque tissue culture plate

1

Luminescence-Based Assay for Kinase Inhibitors in Synchronous Plasmodium falciparum

Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-Luciferase expressing Plasmodium falciparum blood stage parasites were cultured asexually. Assays were performed on synchronous (>90%) ring stage parasites at 2% parasitemia and 5% hematocrit. In triplicate, kinase inhibitors were administered to parasites at 500 nM with vehicle as a non-treatment control (0.5% DMSO in PBS), and Chloroquine administered at 2.5 μM as a positive control. At 44 h post treatment, 30 μL of the assay culture was transferred to a 96-well white flat-bottom opaque tissue culture plate (Beckton Dickinson, Franklin Lakes, New Jersey). Using a Berthold LB960 XS3 microplate (Berthold Technologies, Wildbad, Germany) an equal volume of Bright-Glo luciferase reagent (Promega, Madison, WI) was added to parasites and resultant luminescence was measured. Experimental data is representative of three independent experiments.
+ Open protocol
+ Expand
2

Plasmodium falciparum Luciferase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-Luciferase expressing Plasmodium falciparum blood stage parasites [21 (link)] were cultured asexually. Assays were performed on synchronous (>90%) ring stage parasites at 2% parasitemia and 5% hematocrit. In triplicate, Erastin was administered to parasites at different titrations with vehicle as a non-treatment control (1% DMSO in complete media), and Chloroquine administered at 2.5 µM as a positive control. At 44 h post treatment, 30 µL of the assay culture was transferred to 96-well white flat-bottom opaque tissue culture plate (Beckton Dickinson, Franklin Lakes, NJ). Using a Berthold LB960 XS3 microplate (Berthold Technologies, Wildbad, Germany) an equal volume of Bright-Glo luciferase reagent (Promega, Madison, WI) was added to parasites and resultant luminescence was measured. Experimental data is representative of three biological replicates.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!