Beta cyano l alanine bca

PX-12 and anti-mouse antibody against CTH were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Beta-cyano-L-Alanine (BCA) was from Cayman Chemical (Ann Arbor, MI, USA). siRNAs of CTH1 and CTH2 were purchased from QIAGEN (Tokyo, Japan). 4-acetamido-4'-maleimidylstilbene-2, 2'-disulfonic acid (AMS) was bought from Life Technologies (Eugene, OR, USA). Alexa 680 C2 maleimide was from Thermo Scientific (Rockford, IL). Anti-rabbit antibodies against Trx1 (C63C6), horseradish peroxidase (HRP)-conjugated anti-rabbit or mouse IgG were bought from Cell Signaling Technology (Danvers, MA, USA). Sodium hydrosulfide hydrate (NaHS), L-cysteine hydrochloride, DL-Propargylglycine (PAG), recombinant Trx (rTrx) and all other chemicals were from Sigma (Tokyo, Japan).

OxyBlot protein oxidation detection kit was purchased from Merck Millipore (EMD Millipore, Billerica, MA, USA) and SulfoBiotics- HSip-1 DA from Dojindo Laboratories (Kumamoto, Japan). The generation of superoxide anion (O₂^•−) and ROS were detected using a commercially available kit from Enzo (Tokyo, Japan). Dimedone and acrolein were purchased from Tokyo Chemical Industry (Tokyo, Japan). The anti-cysteine sulfenic acid antibody was from Millipore (Burlington, MA, USA). Beta-cyano L-Alanine (BCA) was from Cayman Chemical (Ann Arbor, MI, USA). Anti-CSE, anti-CBS, and anti-3MPST antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-actin and anti-P-P38 antibodies, as well as horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG, were purchased from Cell Signaling, Inc. (Danvers, MA, USA). Alexa 680 Fluor C2 maleimide was from Thermo Scientific (Rockford, IL, USA). 17β-estradiol (E2), Sodium hydrosulfide hydrate (NaHS), L-cysteine hydrochloride, DL-Propargylglycine (PAG), glutathione (GSH), anti-Cx43, and all other chemicals were from Sigma (Tokyo, Japan).