For histological analysis, the following primary antibodies (Abs) were employed: anti-vitronectin Abs (ab45139, Abcam, Cambridge, MA, and MAB38751, R&D Systems, Inc., Minneapolis, MN), anti-thrombospondin 1 Abs (Ab-11, Thermo Fisher Bio-scientific, Hudson, NH, and ab1823, Abcam), anti-CD45 Abs (ab10558, Abcam, and M0701, DAKO, Carpinteria, CA, USA). Additionally, for B220+CD11c+NK1.1+ NK cell sorting, the following antibodies were used; PE/Cy7 anti-mouse B220 (103222, BioLegends), PE anti-mouse CD11c (117308, BioLegends), APC anti-mouse NK1.1 (108710, BioLegends), and those isotype control antibodies, PE/Cy7 Rat IgG2a, κ (400521, BioLegends), PE American Hamster IgG (12-4888-81, Thermo Fisher), APC Mouse IgG2a κ (550882, BD Biosciences).
Apc mouse igg2a κ
Manufactured by BD
The APC Mouse IgG2a κ is a laboratory reagent used for flow cytometry and other immunological applications. It is an allophycocyanin (APC)-conjugated monoclonal antibody that targets the IgG2a isotype of mouse immunoglobulins with a kappa light chain. This product is designed to be used as a detection or staining reagent in various research procedures.
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Lab products found in correlation
Pe anti mouse cd11c, by BioLegend (1 mentions)
M0701, by Agilent Technologies (1 mentions)
Ab1823, by Abcam (1 mentions)
Ab45139, by Abcam (1 mentions)
Ab10558, by Abcam (1 mentions)
Mab38751, by R&D Systems (1 mentions)
Apc anti mouse nk1, by BioLegend (1 mentions)
Facsdiva software, by BD (1 mentions)
Apc cy7 mouse igg1κ, by BD (1 mentions)
2 protocols using apc mouse igg2a κ
1
Histological Analysis and NK Cell Sorting
2
Identifying Monocyte-derived CD83+ Cells
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To distinguish the CD83 + monocyte-derived population, the PBMCs were suspended in 100 μl of PBS and labelled by monoclonal antibodies against the following surface markers: CD14 (BD Biosciences, APC Mouse IgG 2a κ), CD16 (BD Biosciences, APC-Cy7 Mouse IgG 1 , κ), and CD83 (BD Biosciences, PE-Cy7 Mouse IgG 1 , κ). The samples were incubated in the dark for 30 min at room temperature, washed with 2 ml PBS, and centrifuged twice (1500 rpm, 6 min). The cells were resuspended in 400 μl PBS and analyzed by using flow cytometry (LSRII, BD Biosciences, USA). The dot-plots' region including monocyte-derived cells contained typically 4000 of all events. The mean fluorescence intensities (MFI) of CD14, CD16, and CD83 markers were verified. Data were analyzed by FACSDiva software (BD Biosciences, USA). The monocyte-derived cells were identified using expression of CD14/CD16. The cultured CD83 + cells were identified using an SSC/CD83 expression parameter. Gates delineating positive staining were set on the basis of isotype control staining.
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