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Phloretin

Manufactured by Biosynth
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Sourced in United Kingdom

Phloretin is a naturally occurring compound that can be used as a laboratory reagent. It serves as a core functional component in various experimental procedures and analyses.

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Lab products found in correlation

Udp glucose, by Biosynth (2 mentions) Nothofagin, by Biosynth (2 mentions) Naringenin, by Biosynth (1 mentions) Rutin, by Biosynth (1 mentions) Carbosynth, by Biosynth (1 mentions) Ellagic acid, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) Phloroglucinol, by Merck Group (1 mentions) Apigenin, by Biosynth (1 mentions) Naringin, by Biosynth (1 mentions) 4 methylumbelliferone, by Merck Group (1 mentions) Udp disodium salt, by Biosynth (1 mentions) Carboxymethylcellulose, by Merck Group (1 mentions) Citric acid, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions)

4 protocols using phloretin

1

Phenolic Compound Characterization Protocol

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The phenolic standards, rutin (≥ 95% purity, CAS No. 153-18-4), epigallocatechin-3-O-gallate (EGCG) (≥ 95% purity, CAS No. 989-51-5), (+)-naringenin (≥ 98% purity, CAS No. 67604-48-2), naringin (≥ 95% purity, CAS No. 10236-47-2), apigenin (≥ 94% purity, CAS No. 520-36-5), daidzein (≥ 98% purity, CAS No. 486-66-8), phloretin (≥ 98% purity, CAS No. 60-82-2) were purchased from Biosynth® CarboSynth (Bratislava, Slovakia); whereas quercetin (≥ 95% purity, CAS No. 117-39-5), ellagic acid (≥ 96% purity, CAS No. 476-66-4) and phloroglucinol (≥99% purity, CAS No. 108-73-6) - were purchased from Sigma-Aldrich (Madrid, Spain). HPLC-grade methanol, water, and acetonitrile were supplied by Fisher Scientific Chemicals. The enzymes α-amylase (A1031), pepsin from porcine stomach mucosa (P7012), pancreatin from porcine pancreas (P7545), and bile salts (B863) were purchased from Sigma-Aldrich (Taufkirchen, Germany). The rabbit gastric extract (RGE15) with gastric lipase activity was purchased from Lipolytech (Marseille, France). The ethanol was purchased from Merck (Algés, Portugal). A dialysis membrane with a molecular pore size of 3 kDa was purchased from Spectra/Pro (Spectrum Lab, Breda, Netherlands).
Pais A.C., Coscueta E.R., Pintado M.M., Silvestre A.J, & Santos S.A. (2024). Exploring the bioaccessibility and intestinal absorption of major classes of pure phenolic compounds using in vitro simulated gastrointestinal digestion. Heliyon, 10(7), e28894.
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2

Synthesis of UDP-sugar Derivatives

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Chemicals were from Carl Roth (Karlsruhe,
Germany) and Sigma-Aldrich (Vienna, Austria) in highest purity available.
UDP disodium salt, UDP-d-glucose disodium salt, UDP-d-glucuronic acid ammonium salt, 7-amino-4-methylcoumarin, and phloretin
(98%) were from Carbosynth (Berkshire, UK). 4-Methylumbelliferone
(≥98%) was from Sigma-Aldrich.
Jung J., Schmölzer K., Schachtschabel D., Speitling M, & Nidetzky B. (2021). Selective β-Mono-Glycosylation of a C15-Hydroxylated Metabolite of the Agricultural Herbicide Cinmethylin Using Leloir Glycosyltransferases. Journal of Agricultural and Food Chemistry, 69(19), 5491-5499.
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3

Carboxymethyl Cellulose-Based Hydrogel Synthesis

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Carboxymethyl cellulose
(CMC, DSCOOH: 0.9; M.wt: 700 kDa), citric acid (CA ≥
99.5%), and phosphate buffered saline (PBS, pH 7.4) were purchased
from Sigma-Aldrich, Austria. Nanofibrillated cellulose (NFC, 3 wt
%) was obtained from the University of Maine, USA. Phloretin (>98%),
nothofagin (>98%), UDP (97%), HEPES buffer solution, and UDP-glucose
(>98%) were from Carbosynth (Berkshire, UK). Ultrapure water (Milli-Q
system, Millipore, USA; R > 18.18 M Ω cm) was used for the
preparation
of all samples.
Lackner F., Liu H., Štiglic A.D., Bračič M., Kargl R., Nidetzky B., Mohan T, & Kleinschek K.S. (2022). 3D Printed Porous Nanocellulose-Based Scaffolds As Carriers for Immobilization of Glycosyltransferases. ACS Applied Bio Materials, 5(12), 5728-5740.
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4

Cyclodextrin-Mediated Compound Isolation

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ReliSorb SP400 carrier was from Resindion S.R.L. 2‐Hydroxypropyl‐β‐cyclodextrin (>98%), phloretin (>98%), nothofagin (>98%), UDP (97%), and UDP‐glucose (>98%) were from Carbosynth. Unless indicated, all other chemicals were of analytical grade and obtained from Sigma‐Aldrich.
Liu H, & Nidetzky B. (2021). Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin. Biotechnology and Bioengineering, 118(11), 4402-4413.
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