Reactive oxygen species kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Reactive Oxygen Species (ROS) kit is a laboratory tool designed to detect and measure the presence of reactive oxygen species within a sample. It provides a reliable and quantitative method for researchers to assess oxidative stress levels in various biological systems.

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5 protocols using reactive oxygen species kit

Investigating Zhilong Huoxue Tongyu Capsule's Mechanism

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Zhilong Huoxue Tongyu capsule (Med-drug permit no. Chuan Z20070528; Patent number 200810147774.1) was provided by the Preparation Room for TCM at the Affiliated Hospital of TCM, Southwest Medical University. The specific information of antibodies (p-PI3K, PI3K, p-AKT, AKT, Nrf2, HO-1, GPX4, ACSL4, GAPDH) is presented in S1 Table. RNA extraction, reverse transcription, and reaction reagents were purchased from TOYOBO (Shanghai) Biotech Co., Ltd. The BCA protein concentration assay kit and protein lysis buffer were purchased from Beyotime Biotechnology Co., Ltd. The lactate dehydrogenase (LDH) kit, Creatine kinase-MB isoenzyme (CK-MB) kit, malondialdehyde (MDA) kit, superoxide dismutase (SOD) kit, reactive oxygen species (ROS) kit, and ELISA kit were purchased from Jiancheng (Nanjing) Biotech Co., Ltd.

Zhao X., Yang F., Wu H., Fan Z., Wei G., Zou Y., Xue J., Liu M, & Chen G. (2024). Zhilong Huoxue Tongyu capsule improves myocardial ischemia/reperfusion injury via the PI3K/AKT/Nrf2 axis. PLOS ONE, 19(4), e0302650.

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PLGA-Based Nanoparticle Formulation for Anti-inflammatory Effects

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PLGA (Mw = 38–54 kg/mol), poly(vinyl alcohol) (PVA, 86-89% hydrolyzed, low molecular weight), CUR, dichloromethane (DCM), ABC, Triton X-100 and lipopolysaccharide (LPS) were supplied by Sigma-Aldrich (St. Louis, USA). Paraformaldehyde solution (16%) was purchased from Electron Microscopy Science (Hatfield, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Invitrogen (Eugene, USA). Myeloperoxidase (MPO) Kit and Reactive Oxygen Species (ROS) Kit were supplied by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Dextran sulfate sodium (DSS, 36–50 kDa) was purchased from MP Biomedicals (Aurora, USA). Buffered formalin (10%) was received from EMD Millipore (Billerica, USA). Hematoxylin and eosin were supplied by Richard-Allan Scientific (Kalamazoo, USA). All commercial products were used without further purification.

Chen Q., Si X., Ma L., Ma P., Hou M., Bai S., Wu X., Wan Y., Xiao B, & Merlin D. (2017). Oral delivery of curcumin via porous polymeric nanoparticles for effective ulcerative colitis therapy. Journal of materials chemistry. B, Materials for biology and medicine, 5(29), 5881-5891.

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Cecal Mucosa Antioxidant and Inflammatory Analysis

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The broilers were euthanized by cervical dislocation, and the cecal mucosa samples were collected using sterilized surgical tools and stored at –80℃ for further analysis. Commercial kits were utilized to detect cecal mucosa indexes following the manufacturer's instructions. Enzyme activity assay kits were purchased from Jiangsu Meimian Industrial Co., Ltd. (Yancheng, China) for the determination of total antioxidant capacity (TAOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT). Enzyme-linked immunosorbent assay kits were purchased from Jiangsu Meimian Industrial Co., Ltd. (Yancheng, China) for the determination of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin 4 (IL-4), interleukin 6 (IL-6) and interleukin 10 (IL-10), while reactive oxygen species (ROS) kit (chemical fluorescence method) was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The protein concentration of mucosa was measured using a BCA protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Wu F., Zhao M., Tang Z., Wang F., Han S., Liu S, & Chen B. (2024). Curcumin alleviates cecal oxidative injury in diquat-induced broilers by regulating the Nrf2/ARE pathway and microflora. Poultry Science, 103(5), 103651.

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Macroporous Resin for ROS Analysis

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NKA-9 macroporous adsorption resin was purchased from Solarbio Co. (Beijing, China); agar powder, peptone, and tryptone were purchased from Beijing Auboxing Biotechnology Co., Ltd. (Beijing, China); reactive oxygen species (ROS) kit, SOD kit (batch no. 20220307), CAT visible light kit (batch no. 20220330), and total protein quantitative test kit (batch no. 20220416) were purchased from Nanjing JianCheng Bioengineering Institute (Nanjing, China).

Luan Y., Jiang Y., Huang R., Wang X., He X., Liu Y, & Tan P. (2023). Polygonati Rhizoma Polysaccharide Prolongs Lifespan and Healthspan in Caenorhabditis elegans. Molecules, 28(5), 2235.

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Resveratrol's Lipid-Regulating Effects

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Resveratrol was purchased from Yixin Pharm, Inc. (Zhejiang, China); The triglyceride (TG) kit, total cholesterol (TC) kit, low density lipoprotein cholesterol (LDL-C) kit, high density lipoprotein cholesterol (HDL-C) kit, superoxide dismutase (SOD) kit, malonaldehyde (MDA) kit, free fatty acid (FFA) kit, reactive oxygen species (ROS) kit, glutathione (GSH) kit, glutathione peroxidase (GPx) kit, and the coomassie brilliant blue kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China); rabbit anti-mouse p47phox polyclonal antibody was purchased from LifeSpan Biosciences, Inc. (WA, USA); rabbit anti-mouse Sirt1 monoclonal antibody and the gp91phox polyclonal antibody were procured from Abcam, Inc. (Cambridge, UK); Adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and p-HSL (Ser660) antibodies were purchased from Santa Cruz Biotech (Texas, USA); the primary antibodies including rabbit anti-mouse AMPK, p-AMPK (Thr172), FOXO1, and p-FOXO1 (Thr24) were purchased from Cell Signaling Technology (MA, USA); GAPDH antibody was obtained from Boster, Inc. (Wuhan, China); the BCA kit and HRP-labeled goat anti-rabbit secondary antibody were from Beyotime, Inc. (Jiangsu, China).

Zhu W., Chen S., Li Z., Zhao X., Li W., Sun Y., Zhang Z., Ling W, & Feng X. (2014). Effects and mechanisms of resveratrol on the amelioration of oxidative stress and hepatic steatosis in KKAy mice. Nutrition & Metabolism, 11, 35.

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