Trichloroacetic acid

Manufactured by Honeywell

Sourced in Germany

Trichloroacetic acid is a colorless crystalline compound with the chemical formula CCl3COOH. It is a strong carboxylic acid commonly used in analytical and organic chemistry laboratories.

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Lab products found in correlation

4 protocols using trichloroacetic acid

Determination of Lead in Solutions

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Analytical standard solutions of lead (Spectro ECON) were prepared by serially diluting a 1000 mg/L stock calibration standard solution. Reagents used included trichloroacetic acid (5%; TCA) and perchloric acid (extra pure, Riedel-de Haen, Germany), K₂EDTA and ammonium pyrollidine dithiocarbamates (APDC; Merck (Darmstadt), calcium chloride and methyl iso-butyl ketone (MIBK; Sigma Chemical Co).

Gebrie H.A., Tessema D.A, & Ambelu A. (2014). Elevated blood lead levels among unskilled construction workers in Jimma, Ethiopia. Journal of Occupational Medicine and Toxicology (London, England), 9, 12.

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Analytical Grade Chemicals Protocol

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All chemicals were analytical grade. Methanol (MeOH), iron (II)-sulfate-7-hydrate and trichloroacetic acid were obtained from Riedel-de Haën™ (Seelze, Germany) and formic acid, water and acetonitrile (ACN) from Fisher Scientific, OptimaTM (Hampton, NH, USA). Trifluoroacetic acid (TFA), potassium dihydrogen phosphate, tris(hydroxymethyl)aminomethane (Tris) and sodium chloride were bought from Merck (Darmstadt, Germany). Ethanol 96% was bought from Carlo Erba (Peypin, France), gentiopicroside from TransMIT (Hesse, Germany) and potassium chloride from Fluka (Seelze, Germany). Acetylcholinesterase (AChE), acetylcholine iodide (AChI), 5-5′-dithiobis (2-nitrobenzoic acid) (DTNB), HMG-CoA Reductase assay kit, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), butylated hydroxytoluene (BHT), quercetin, rutin and galantamine were obtained from Sigma (Barcelona, Spain). Magnesium chloride hexahydrate, thiobarbituric acid, hydrogen peroxide, sodium hydrogen phosphate and 1-butanol were bought to PanReac (Barcelona, Spain). Sodium hydroxide was obtained from JT Baker, UK and sodium nitroprusside and Griess reagent from VWR Chemicals PL.

Guedes L., Reis P.B., Machuqueiro M., Ressaissi A., Pacheco R, & Serralheiro M.L. (2019). Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies. Molecules, 24(20), 3795.

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Tiamulin Residue Analysis in Food

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The following reagents were used: tiamulin, standard substance (Dr. Ehrenstorfer GmbH—Augsburg, Germany), disodium salt of ethylenediaminetetraacetic acid (Na₂EDTA dihydrate; Sigma—Livonia, MI, USA), anhydrous citric acid p.a. (Panreac—Barcelona, Spain), pure trichloroacetic acid, 99%, (Fluka -France), disodium phosphate anhydrous (Na₂HPO₄) p.a. (Panreac), acetonitrile, methanol, formic acid, 98–100% (Riedel-de Haen—Nottingham, UK), trichloroacetic acid, 20% (m/v).
The extraction solution is McIlvaine–EDTA buffer 0.1 M at pH 4, stable for a week. Standard stock solutions (1000 µg/mL) in methanol were prepared, stable for 6 months at −20 °C. On the day of use, 5 standard calibration solutions (0.05; 0.1; 0.25; 0.5; 1.0 μg/mL) were prepared in control sample extract (matrix) corresponding to 25–500 μg/kg.

Ciucă V.C., Rusănescu C.O, & Safta V.V. (2023). Analysis of Transfer of Tiamulin to Animal Tissue after Oral Administration: An Important Factor for Ensuring Food Safety and Environmental Protection. Pharmaceuticals, 16(3), 387.

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Antioxidant Capacity Evaluation Assays

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The 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), aluminium(III) chloride, and quercetin were obtained from Alfa Aesar (Tewksbury, MA, USA). Vitamin C was purchased from Acros (Geel, Belgium). Sodium hydroxide was provided by Choneye Chemical Co. (Taipei, Taiwan). Ethanol was procured from Echo Chemical Co. (Miaoli, Taiwan). Potassium ferricyanide was purchased from First Chemical Co. (Pascagoula, MS, USA). Folin-Ciocâlteu’s (Folin) reagent was obtained from Fisher Scientific (Loughborough, Leicestershire, UK). Gallic acid (GA) was provided by Fluka (Neu-Ulm, Germany). Iron(III) chloride hexahydrate was purchased from J.T. Baker (Phillipsburg, NJ, USA). Sodium carbonate, sodium phosphate dibasic dihydrate, and trichloroacetic acid were obtained from Riedel-de Haën (Seelze, Germany). Sodium phosphate monobasic was provided by Shimakyu’s Pure Chemical Co. (Osaka, Japan). Potassium perdisulfate was procured from Showa Chemical Co. (Tokyo, Japan). Di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium and sodium nitrite were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Wu C.T., Wang W.H., Lin W.S., Hu S.Y., Chen C.Y., Chang M.Y., Lin Y.S, & Li C.P. (2021). Effects of Different Chenopodium formosanum Parts on Antioxidant Capacity and Optimal Extraction Analysis by Taguchi Method. Materials, 14(16), 4679.

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