PDGF-BB was purchased from PeproTech (Shanghai, China). Alginate (Agn, A0682) was obtained from Sigma (St Louis, United States). CCl4, ALT, LDH, Hyp and AST kits were purchased from the Nanjing Jiancheng Bioengineering (Nanjing, China). ELISA kits were purchased from Boyun Biotechnology (Shanghai, China). Primary antibodies were purchased from Cell Signaling Technologies (MA, USA). Western blot reagents were purchased from Beyotime (Shanghai, China). RT-PCR primers were purchased from Sangon Biotech (Shanghai, China). Other reagents were obtained from Takara Bio (Shiga, Japan).
Western blot reagents
Manufactured by Beyotime
Sourced in China
Western blot reagents are a set of products used in the Western blot technique, a widely-used analytical method in molecular biology and biochemistry. The core function of these reagents is to facilitate the detection and quantification of specific proteins within a complex sample.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Pdgf bb, by Thermo Fisher Scientific (1 mentions)
Rt pcr primers, by Sangon (1 mentions)
Calf serum, by Sartorius (1 mentions)
Cck 8 reagent, by Apexbio (1 mentions)
Mccoy s 5a medium, by Sartorius (1 mentions)
Si ezh2, by RiboBio (1 mentions)
Si nc, by RiboBio (1 mentions)
El transfection reagent, by Transgene (1 mentions)
Trizol kit, by Takara Bio (1 mentions)
Total rna extraction kit, by Tiangen Biotech (1 mentions)
Sw620, by Procell (1 mentions)
Chloroform, by Dingguo (1 mentions)
Restriction endonuclease, by Takara Bio (1 mentions)
La taq dna polymerase, by Takara Bio (1 mentions)
T4 dna ligase, by Takara Bio (1 mentions)
Il 1β, by MedChemExpress (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did, by Beyotime (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Beyotime (1 mentions)
Indocyanine green icg, by Maclin Biochemical Technology (1 mentions)
5 protocols using western blot reagents
1
Liver Injury Evaluation Protocol
2
Investigating Colon Cancer Cell Apoptosis
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McCoy’s 5A medium and calf serum were obtained from Biological Industries, Israel; CCK-8 reagents were obtained from APExBIO, USA; qRT-PCR kits were obtained from Tiangen Biotech (Beijing, China) Co., Ltd.; PCR primers were synthesized in Sangon Biotech (Shanghai, China) Co., Ltd.; Western Blot reagents were obtained from Beyotime Biotechnology, China; rabbit anti-human monoclonal antibodies (first antibodies) were obtained from Affinity Biosciences, USA; rabbit anti-human GAPDH monoclonal antibodies (first antibodies) were obtained from Hangzhou Huabio, China; horseradish peroxidase (HRP)-labeled sheep anti-rabbit polyclonal antibodies were obtained from Sera Care Life Sciences, USA; cell apoptosis assay kits were obtained from Nanjing Signalway Antibody, China; Matrigel gel were obtained from Beijing Solarbao Science and Technology Co., Ltd., China; transfection reagents, si-EZH2 and si-NC were synthesized by Ribobio, China; colon carcinoma cell strains HCT-116 were obtained from Shanghai Biomedical, China; HT29 cell strains were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences.
3
Investigating lncRNA in Colon Cancer
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The human colon cancer cell line SW620 was obtained from Wuhan Procell Life Science & Technology Co., Ltd, and hBD-1 was purchased from Sino Biological, Inc. (China). The total RNA extraction kit was purchased from TIANGEN Biotech (Beijing) Co., Ltd. The Trizol kit was acquired from Takara Bio Inc. (Japan), and chloroform was purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd. EL Transfection Reagent was purchased from TransGen Biotech Co., Ltd (Beijing). Additionally, si-lncRNA (Suzhou Gemma Biotechnology Co., Ltd.), BCA kit (Shanghai Epizyme Biotech Co., Ltd.), and Western blot reagents (Beyotime Biotech. Inc., Shanghai) were used. The antibody was purchased from Cell Signaling Technology Inc. (United States).
4
Unnatural Amino Acid Incorporation
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Restriction endonucleases, LA Taq DNA polymerase, and T4 DNA ligase were purchased from Takara Company (Dalian, China). All primers for PCR amplification were synthesized by Sangon Biotech (Shanghai, China) Co. Ltd. and listed in Table 1 . The unnatural amino acid p-propargyloxyphenylalanine (pPpa) was provided by Amatek Chemical Company. Western-blot reagents were purchased from Beyotime Biotechnology (Shanghai, China).
5
Multimodal Imaging and Molecular Regulation
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3,3′-dioctadecyloxacarbocyanine perchlorate
(DiO), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
(DiI), 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate
Salt(DiD), ROS flow kit, and Western blot reagents were purchased
from Beyotime Biotechnology (China). The cytokines used in this study
(TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)),
lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress
(MCE, China). Indocyanine green (ICG) was obtained from Macklin Inc.
(China). The apoptosis flow kit and cell counting kit-8 (CCK-8) cell
viability assay kit were purchased from Vazyme Biotech Co., Ltd. (China).
The J774A.1 cell line was purchased from Procell Life Science &
Technology Co., Ltd. (China). Lipofectamine 3000, PBS, media, serum,
and ELISA kits required for cell culture were purchased from Thermo
Fisher Scientific Inc. (Waltham, MA, USA). The PCR-related reagents
were purchased from Yeasen Biotechnology Co., Ltd. (China). The animals
needed for the experiment were purchased from the Laboratory Animal
Center of Huazhong University of Science and Technology.
(DiO), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
(DiI), 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate
Salt(DiD), ROS flow kit, and Western blot reagents were purchased
from Beyotime Biotechnology (China). The cytokines used in this study
(TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)),
lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress
(MCE, China). Indocyanine green (ICG) was obtained from Macklin Inc.
(China). The apoptosis flow kit and cell counting kit-8 (CCK-8) cell
viability assay kit were purchased from Vazyme Biotech Co., Ltd. (China).
The J774A.1 cell line was purchased from Procell Life Science &
Technology Co., Ltd. (China). Lipofectamine 3000, PBS, media, serum,
and ELISA kits required for cell culture were purchased from Thermo
Fisher Scientific Inc. (Waltham, MA, USA). The PCR-related reagents
were purchased from Yeasen Biotechnology Co., Ltd. (China). The animals
needed for the experiment were purchased from the Laboratory Animal
Center of Huazhong University of Science and Technology.
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