Elambda dad

Analysis of flavonoid composition was made using a mobile phase consisting of 70% ultrapure water with 0.1% formic acid (A), 30% acetonitrile (B), and 5 μL injected into a UHPLC system. Chromatographic conditions were described elsewhere [46 (link)].
Briefly, the analysis was performed on an Acquity UHPLC System (Waters Ges.m.b.H., Wien, Austria) coupled with an eLambda DAD (Waters) and a high-resolution Fourier transform Exactive Orbitrap mass spectrometer (Thermo Fischer Scientific, Inc., Barrington, IL, USA), equipped with a HESI-II probe for electrospray ionization (ESI) and a collision cell (HCD), using a BEH Shield C₁₈ column (150 × 2.1 mm, 1.7 μm particle size; Waters). The MS data were processed using Xcalibur software (Thermo Fischer Scientific). The peak identity was ascertained by evaluating the accurate mass, the fragments obtained in the collision cell, and the online UV spectra (200–400 nm).

Identification of flavan-3-ols was performed following Valderrama-Soto et al. (2021) (link). The analysis was done on an Acquity UHPLC system (Waters, Milford, MA, United States), coupled with an eLambda DAD (Waters, Milford, MA, United States) and a High-Resolution Fourier Transform Orbitrap mass spectrometer, using the Extractive model (Thermo Scientific, Rodano, Italy), equipped with a HESI-II probe for ESI, operating in a positive mode and a collision cell (HCD). The MS data were processed using Xcalibur software (Thermo Scientific, Rodano, Italy). The peak identity was ascertained by evaluating the accurate mass, the fragments obtained in the collision cell, and by the online UV spectra (200–600 nm).