Freesia “Shiny Gold”, “Yvonne”, “10C3-894” and “10C3-424” were harvested at anthesis and cut inflorescence and were placed immediately in deionized water under laboratory condition at 25 °C. For headspace solid-phase microextraction (HS-SPME), individual flowers were cut from the inflorescence and weighed (2 g). Samples were placed inside a 20 mL glass vial and sealed with polytetrafluoroethylene (PTFE) silicone septum (Agilent Technologies, Inc., Santa Clara, CA, USA). Volatile compounds were adsorbed onto 80 μm divinylbenzene–carboxen–polydimethylsiloxane fiber [DVB/C-WR/PDMS] (PAL SYSTEM, Switzerland) for 10 min at 30 °C. The SPME syringe was injected into the injector port of the GC–MS for desorption.
Ptfe silicone septum
Manufactured by Agilent Technologies
Sourced in United States
The PTFE/silicone septum is a component used in various laboratory equipment. It serves as a gas-tight seal, allowing the introduction of samples or reagents through a small aperture while maintaining the integrity of the system.
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Lab products found in correlation
Dvb car pdms, by Merck Group (2 mentions)
Agilent 5977b, by Agilent Technologies (2 mentions)
Gc system, by Agilent Technologies (1 mentions)
Supelcowax 10, by Merck Group (1 mentions)
Agilent masshunter qualitative analysis, by Agilent Technologies (1 mentions)
Supelcowax 10 column, by Merck Group (1 mentions)
4 protocols using ptfe silicone septum
1
Headspace Analysis of Freesia Volatiles
2
Hexanal Analysis in Walnut Samples
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Hexanal was extracted using solid-phase microextraction (SPME). Samples of walnut grounded (3 g ± 0.1 g), spiked with 4-methyl-2-pentanol as internal standard (2.5 mg/kg), was weighed into a 20 mL glass vial and sealed with a PTFE/silicone septum (Agilent Technologies, Palo Alto, CA, USA). After 10 min in agitation mode, at 40 °C, a solid-phase microextraction (SPME) fiber (DVB/CAR/PDMS, Sigma-Aldrich, St. Louis, MO, USA) was exposed to the sample headspace for 40 min for volatile extraction. The hexanal analysis was performed in triplicate using a GC system (Agilent Technologies, Palo Alto, CA, USA) that comprise an autosampler (Agilent PAL RSI 85), a gas chromatograph (GC Agilent 7820A), and a mass spectrometer (Agilent 5977B) that included an electron impact source and a quadrupole analyzer. A Supelcowax 10 (30 m × 0.25 mm × 0.25 μm, Sigma-Aldrich, St. Louis, MO, USA) was used for compounds separation. Helium was used as carrier gas at a flow rate of 1 mL/min. GC oven temperature started at 40 °C and ramped at 3 °C/min after 10 min to the final temperature of 200 °C. The data were recorded and analyzed using Agilent MassHunter Qualitative Analysis. Hexanal was identified using the NIST 08 Mass Spectral Library and via comparison with the standard (Sigma-Aldrich, St. Louis, MO, USA).
3
Raspberry Antioxidant Preservation Protocol
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Replicates of 0.5 g of raspberry frozen grinded samples, stored at −80°C, were weighed into 20 mL glass vials with screw cap with PTFE/silicone septum (Agilent, Cernusco sul Naviglio, Italy). 0.5 mL of an antioxidant solution (0.5 mL of deionized water, 200 mg of sodium chloride, 2.5 mg of ascorbic acid, and 2.5 mg of citric acid) was added on top according to Farneti et al., 2017b (link).
4
Extraction and Analysis of Volatile Compounds
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Volatile compounds from kernel and oil were extracted by solid-phase microextraction (SPME) [13 (link)]. Briefly, 3.0 g of grounded kernel flour or extracted oil was mixed with 2.5 mg/kg of 4-methyl-2-pentanol (internal standard), stirred for 10 min at 40 °C until equilibration in a 20 mL glass vial with a PTFE/silicone septum (Agilent Technologies, Palo alto, CA, USA). Next, a solid phase microextraction (SPME) fiber (DVB/CAR/PDMS, Sigma-Aldrich, St. Louis, MO, USA) was exposed to the sample headspace for 40 min. Next, the volatile compounds were analyzed with a GC system (Agilent Technologies) comprising an autosampler (Agilent PAL RSI 85), gas chromatograph (GC Agilent 7820A) and mass spectrometer (Agilent 5977B) designed with an electron impact source and quadrupole analyzer. Volatile compounds were separated with a Supelcowax 10 column (30 m × 0.25 mm × 0.25 µm, Sigma-Aldrich, USA) using helium as a carrier gas at a flow rate of 1 mL/min. GC oven temperature was started at 40 °C and increased by 3 °C/min after 10 min, until it reached a final temperature of 200 °C. Volatiles were identified by two methods, including the use of NIST 08 Mass Spectral Library and comparison of their retention time and mass spectrum to their respective standards. Results were expressed as µg of internal standard per kg of kernel or oil sample.
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