C57BL/6J mice were obtained from The Jackson Laboratory and all mouse strains used in this study were backcrossed to female C57BL/6J breeders for more than ten generations. Snx5−/− mice were generated by the UC DAVIS Knockout Mouse Project (KOMP) Repository (https://www.komp.org/ ) and have been previously described23 (link). Genomic DNA from mice was prepared from mouse tail using DirectPCR Lysis Reagent (Viagen Biotech) and Proteinase K (Roche). Mouse genotypes were determined by PCR using TaKaRa SapphireAmp Fast PCR Master Mix (Clontech) and the following primer pairs: Snx5 WT-1, 5’-GCTTTGCCTCTGATTTGGATCTCC-3’; Snx5 WT-2, 5’-CTAAGACAATAAAACCCACCGGGCG-3’; Snx5 KO-1, 5’-GACATGAGCACACTGTTAGTGCAGG-3’; Snx5 KO-2, 5’-GAGATGGCGCAACGCAATTAATG-3’. The PCR conditions are as follows: 1 min at 94°C, 35 cycles of amplification stage (5 sec at 98°C, 5 sec at 55°C and 5 sec at 72°C) and 5 min at 72°C. Snx5+/+ genomic DNA yields a 405-bp band; Snx5−/− genomic DNA yields a 386-bp band; Snx5+/− genomic DNA yields both a 405-bp band and a 386-bp band.
Takara sapphireamp fast pcr master mix
Manufactured by Takara Bio
TaKaRa SapphireAmp Fast PCR Master Mix is a ready-to-use solution for performing fast and efficient PCR amplification. It contains all the necessary components, including a high-fidelity DNA polymerase, dNTPs, and reaction buffer, optimized for rapid thermal cycling.
Automatically generated - may contain errors
2 protocols using takara sapphireamp fast pcr master mix
1
Genotyping Snx5 Knockout Mice
2
Genotyping Snx5 Knockout Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J mice were obtained from The Jackson Laboratory and all mouse strains used in this study were backcrossed to female C57BL/6J breeders for more than ten generations. Snx5−/− mice were generated by the UC DAVIS Knockout Mouse Project (KOMP) Repository (https://www.komp.org/ ) and have been previously described23 (link). Genomic DNA from mice was prepared from mouse tail using DirectPCR Lysis Reagent (Viagen Biotech) and Proteinase K (Roche). Mouse genotypes were determined by PCR using TaKaRa SapphireAmp Fast PCR Master Mix (Clontech) and the following primer pairs: Snx5 WT-1, 5’-GCTTTGCCTCTGATTTGGATCTCC-3’; Snx5 WT-2, 5’-CTAAGACAATAAAACCCACCGGGCG-3’; Snx5 KO-1, 5’-GACATGAGCACACTGTTAGTGCAGG-3’; Snx5 KO-2, 5’-GAGATGGCGCAACGCAATTAATG-3’. The PCR conditions are as follows: 1 min at 94°C, 35 cycles of amplification stage (5 sec at 98°C, 5 sec at 55°C and 5 sec at 72°C) and 5 min at 72°C. Snx5+/+ genomic DNA yields a 405-bp band; Snx5−/− genomic DNA yields a 386-bp band; Snx5+/− genomic DNA yields both a 405-bp band and a 386-bp band.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!