The photosynthetic characteristics of leaves were measured at 0, 24, 48, and 72 h after treatment using a ciras-3 portable photosynthetic instrument (PPSystems, United Kingdom). Net Pn of leaves was measured at 9:00–11:00, and Fv/Fm was measured with a fluorometer (Handy PEA, Hansatech, United Kingdom).
Ciras 3 portable photosynthetic instrument
Manufactured by PP Systems
Sourced in United Kingdom
The Ciras-3 is a portable photosynthetic instrument designed to measure the gas exchange of plants. It provides accurate measurements of photosynthesis, respiration, and other related parameters in a compact and portable package.
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Lab products found in correlation
3 protocols using ciras 3 portable photosynthetic instrument
1
Photosynthetic Response to Treatment
2
Leaf Physiological Responses to Low-Temperature Stress
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The leaves were harvested after nine days of treatment. The relative water content of the leaves was determined as described by Wang et al. [49 ]. Leaf electrolyte leakage was determined using an electrical conductivity meter (DDSJ-308 F, Shanghai, China) according to the method described by Song et al. [50 (link)]. Malondialdehyde content was measured as described by Chen et al. [54 ]. Nine days after the low-temperature treatment started, the net photosynthetic rate (Pn) was measured on the penultimate leaves using a CIRAS-3 portable photosynthetic instrument (PP Systems, USA) [52 ]. The fluorescence parameters Fv/Fm and chlorophyll (Chl) fluorescence images of the penultimate leaves were analyzed after 30 min of dark adaptation using fluorescence measurements with a HITACHI F-7000 fluorescence spectrophotometer (Tokyo, Japan) [53 ]. Chlorophyll (Chl) content was analyzed as previously described by Li et al. [54 ].
3
Photosynthetic Measurements of Tree Saplings
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The net
photosynthetic rate (Pn), stomatal conductance
(Gs), transpiration rate (Tr), and intercellular CO2 concentration (Ci) of sapling leaves were measured with a Ciras-3
portable photosynthetic instrument (PP Systems, UK) from 9:00 a.m.
to 11:00 a.m. on a sunny day in mid-July of 2018 and 2019. Three plants
were randomly selected for these measurements, and three mature leaves
(3rd to 5th leaves from the top) were measured per plant. Photosynthetic
measurements were made at a light intensity of 1000 ± 50 μmol
m–2 s–1, a CO2 concentration
of 360 ± 20 μL L–1, and a temperature
of 26 ± 1 °C.83 (link)
photosynthetic rate (Pn), stomatal conductance
(Gs), transpiration rate (Tr), and intercellular CO2 concentration (Ci) of sapling leaves were measured with a Ciras-3
portable photosynthetic instrument (PP Systems, UK) from 9:00 a.m.
to 11:00 a.m. on a sunny day in mid-July of 2018 and 2019. Three plants
were randomly selected for these measurements, and three mature leaves
(3rd to 5th leaves from the top) were measured per plant. Photosynthetic
measurements were made at a light intensity of 1000 ± 50 μmol
m–2 s–1, a CO2 concentration
of 360 ± 20 μL L–1, and a temperature
of 26 ± 1 °C.83 (link)
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