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Anti hdm ige kits

Manufactured by Chondrex

The Anti-HDM IgE kits are laboratory equipment used to detect and quantify IgE antibodies specific to house dust mites (HDM). The kits provide a standardized method for measuring HDM-specific IgE levels, which can be an important indicator of allergic sensitization.

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2 protocols using anti hdm ige kits

1

Quantifying IgG, IgE, and NP-specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For total IgG and IgE levels in serum, Maxisorp (Nunc) plates were coated with anti-mouse Ig (SouthernBiotech) or anti-IgE (BD Biosciences, R35–72) respectively, and serum was incubated. Alkaline-phosphatase secondary reagents (SouthernBiotech) were added, followed by Phosphatase substrate (Sigma), and plates were read on a plate reader (Spectramax). For NP-specific antibody ELISAs, Maxisorp plates were coated with NP-BSA (Biosearch Technologies), followed by secondary reagents above. Anti-HDM IgE kits (Chondrex) were used for HDM specific antibody quantification.
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2

Quantifying IgG, IgE, and NP-specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For total IgG and IgE levels in serum, Maxisorp (Nunc) plates were coated with anti-mouse Ig (SouthernBiotech) or anti-IgE (BD Biosciences, R35–72) respectively, and serum was incubated. Alkaline-phosphatase secondary reagents (SouthernBiotech) were added, followed by Phosphatase substrate (Sigma), and plates were read on a plate reader (Spectramax). For NP-specific antibody ELISAs, Maxisorp plates were coated with NP-BSA (Biosearch Technologies), followed by secondary reagents above. Anti-HDM IgE kits (Chondrex) were used for HDM specific antibody quantification.
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