Sc 17829

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-17829 is a lab equipment product offered by Santa Cruz Biotechnology. It is a device used for the detection and analysis of specific biomolecules in a sample.

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Lab products found in correlation

6 protocols using sc 17829

Immunofluorescence Analysis of Focal Adhesion Proteins

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After 4% PFA fixation, cells were blocked by 5% bovine serum albumin (BSA, A7906, Sigma) in DPBS containing 0.1% Triton X-100 for 1 h, followed by the incubation of primary antibodies against integrin beta 1 (12G10, ab30349, Abcam, 1:200), paxillin (Y113, ab32048, Abcam, 1:200), CD49c (integrin alpha 3, ASC-1, MA5-28565, Invitrogen, 1:50), talin 1 (8D4, ab157808, Abcam, 1:100), vinculin (EPR8185, ab129002, Abcam, 1:100), FAK (#3285, Cell signaling Technology, 1:200), α-actinin (H-2, sc-17829, Santa Cruz Biotechnology, 1:200), and Phospho-Myosin Light Chain 2 (Thr18/Ser19) (pMLC, #3674, Cell Signaling Technology, 1:100) in 1% BSA for overnight at 4 °C. The samples were washed twice with PBS before applying fluorescence-conjugated secondary antibodies, including Goat Anti-Mouse lgG H&L (Alexa Fluor® 488) (ab150113, Abcam, 1:1000), Goat Anti-Rabbit lgG H&L (Alexa Fluor® 488) (ab150077, Abcam, 1:1000), Goat Anti-Rabbit lgG H&L (Alexa Fluor® 568) (ab175471, Abcam, 1:1000), Donkey Anti-Rabbit lgG H&L (Alexa Fluor® 647) (ab150075, Abcam, 1:1000), Goat Anti-Mouse lgG H&L (Alexa Fluor® 568) (ab175473, Abcam, 1:1000), Goat Anti-Mouse lgG H&L (Alexa Fluor® 647) (ab150115, Abcam, 1:1000), in 1% BSA with or without dye-conjugated phalloidin and DAPI (R37606, Invitrogen) at room temperature for 45 min.

Hu X., Roy S.R., Jin C., Li G., Zhang Q., Asano N., Asahina S., Kajiwara T., Takahara A., Feng B., Aoki K., Xu C, & Zhang Y. (2022). Control cell migration by engineering integrin ligand assembly. Nature Communications, 13, 5002.

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Antibody Validation for Hepatitis C Study

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Breifly, 4-PBA and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Daclatasvir (DAV) was purchased from MedChem Express (MedChem Express, Monmouth Junction, NJ, USA). Antibodies used in this study include mouse anti-NS5A; sc-65458, mouse anti-NS3; sc-69938, mouse anti-a-actinin; sc-17829 rabbit anti-hVAP-33; sc-98890, anti-grp78;sc-13968, rabbit anti-PERK; sc-13073, rabbit anti-IRE1a; sc-20790, mouse anti-actin; sc-8432 (Santa cruz, Dallas, TX, USA), rabbit anti-hepcidin; ab75883, rabbit anti-H3K9ac; ab10812, rabbit anti-H3K27ac; ab4729, mouse anti-RNA pol III; ab817, rabbit anti-CypB; ab16045, (abCAM, Canbridge, UK), rabbit anti-OSBP; #11096-1-AP (proteintech, Rosemont, IL, USA), rabbit anti-VAP-B; NBP1-89112 (NOVUS, Centennial, CO, USA), mouse anti-OCH1E5; MBS370077, rabbit anti-NS5A (For Immunohistochemistry); MBS485095 (mybiosource, San Diego, CA, USA). Secondary antibodies used are goat anti-mouse IgG; M32607, goat anti-rabbit IgG; 31460, Alexa Fluor 488 goat anti-rabbit IgG; A-11034, Alexa Fluor 488 goat anti-mouse IgG; A-11001, Alexa Fluor 594 goat anti-mouse IgG; A-11005, Alexa Flour 594 goat anti-rabbit IgG; A-11012 (Invitrogen, Waltham, MA, USA).

Kim K., Lee Y.S., Jeong S., Kim D., Chon S., Pak Y.K., Kim S., Ha J., Kang I, & Choe W. (2020). A Small Molecule, 4-Phenylbutyric Acid, Suppresses HCV Replication via Epigenetically Induced Hepatic Hepcidin. International Journal of Molecular Sciences, 21(15), 5516.

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C7 and α-actinin Quantification

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Cell lysates were collected from the cell culture plate with lysis buffer using a cell scraper. Primary antibodies: rabbit anti-C7 (ab93350, Abcam, 1:500) and mouse anti-α-actinin (sc-17829, Santa Cruz, 1:1,000). Secondary antibodies: goat anti-mouse IgG HRP and goat anti-rabbit IgG HRP (BioRad, 1:5,000). Quantification was performed using ImageJ.

Thompson E.L., Pickett-Leonard M., Riddle M.J., Chen W., Albert F.W, & Tolar J. (2022). Genes and compounds that increase type VII collagen expression as potential treatments for dystrophic epidermolysis bullosa. Experimental dermatology, 31(7), 1065-1075.

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Protein Expression Analysis in Glioma Cells

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The glioma cell lines (including U87MG, U118MG, and LNZ308) were washed three times with PBS and lysed in RIPA buffer (100 mM Tris-HCl in pH 8.0, 0.1% SDS, 150 mM NaCl, and 1% Triton 100). The protein lysates (20–40 µg, depending on the concentration) were separated by 10% SDS-PAGE and analyzed by immunoblotting with antibodies against polyclonal rabbit anti-human CKAP2L (HPA039407, Sigma–Aldrich, St. Louis, MO, USA) and anti-GAPDH (sc-47724, Santa Cruz, CA, USA) antibodies and monoclonal mouse anti-ACTN (sc-17829, Santa Cruz, USA). After repeating the experiments, the quantification was performed using ImageJ (National Institutes of Health, Bethesda, MD, USA).

Li Y.F., Tsai W.C., Chou C.H., Huang L.C., Huang S.M., Hueng D.Y, & Tsai C.K. (2020). CKAP2L Knockdown Exerts Antitumor Effects by Increasing miR-4496 in Glioblastoma Cell Lines. International Journal of Molecular Sciences, 22(1), 197.

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Immunoblotting Analysis of INTS9 Expression

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We washed the cell lines with PBS and lysed them in RIPA buffer (100 mM Tris-HCl in pH 8.0, 150 mM NaCl, 0.1% SDS, and 1% Triton 100). We separated the protein lysates (20–40 µg, based on the concentrations) using 10% SDS-PAGE and analyzed them through immunoblotting with antibodies against polyclonal rabbit anti-GAPDH (sc-47,724, Santa Cruz), anti-INTS9 (Atlas antibodies #HPA007674), and the monoclonal mouse anti-ACTN (sc-17,829, Santa Cruz). We repeated the experiment and quantified the images using the ImageJ software (National Institutes of Health, USA).

Lin Y.C., Chang P.C., Hueng D.Y., Huang S.M, & Li Y.F. (2023). Decoding the prognostic significance of integrator complex subunit 9 (INTS9) in glioma: links to TP53 mutations, E2F signaling, and inflammatory microenvironments. Cancer Cell International, 23, 154.

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Western Blot Analysis of DNA Methylation Regulators

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Cells were lysed with lysis buffer (GenDEPOT). Equal amounts of protein were resolved using 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the resolved proteins were transferred to nitrocellulose membranes. The membranes were blocked via incubation with 5% non-fat dry milk in PBS containing 0.1% Tween-20 (PBST) for 1 h and then incubated with primary antibodies overnight at 4 °C. After three washes with PBST, the blots were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (GenDEPOT) for 30 min at room temperature. Protein bands were detected using Western Lightning Plus ECL (Perkin Elmer, Waltham, MA, USA) and imaged with an Amersham Imager 600 (GE Healthcare, Piscataway, NJ, USA). Primary antibodies against DNMT1 (ab13537), DNMT3a (ab13888), DNMT3b (ab13604), MBD2 (ab38646), and MeCP2 (ab2828) were obtained from Abcam (Abcam, Cambridge, UK), while a primary antibody against α-actinin (sc-17829) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Kim D.Y., Shin D.Y., Oh S., Kim I, & Kim E.J. (2024). Gene Expression and DNA Methylation Profiling Suggest Potential Biomarkers for Azacitidine Resistance in Myelodysplastic Syndrome. International Journal of Molecular Sciences, 25(9), 4723.

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