UV spectra were obtained using a Shimadzu Pharmaspec-1700 UV-Visible spectrophotometer. Optical rotations were measured with a Jasco-DIP-180 polarimeter (JASCO, Inc., Tokyo, Japan). Infrared spectra were recorded on a Perkin-Elmer-983G FT-IR spectrophotometer (PerkinElmer Ltd., Bucks, UK). 1D- and 2D-NMR spectra were measured with a Varian-Unity-Plus-400 spectrometer or a Bruker DRX-500 FT-NMR spectrometer with tetramethylsilane (TMS) as the internal standard (Bruker Instruments, Karlsruhe, Germany). EI-MS and HR-EI-MS were measured with a JEOL SX-102A mass spectrometer and a Finnigan TSQ-46C mass spectrometer, respectively (Finnigan MAT, Inc., San Jose, CA, USA). Column chromatography was performed using Merck Si gel (230–400 mesh; Merck & Co., Inc., White House Station, NJ, USA), and TLC (thin-layer chromatography) analysis was carried out using aluminum pre-coated Si plates (Silica Gel 60 F-254; Merck & Co., Inc.); the spots were detected by spraying with 5% H2SO4 and then heating at 100 °C. Semi-preparative HPLC was performed using a normal phase column (LiChrosorb Si 60, 7 μm, 250 mm × 10 mm; Merck & Co., Inc.) on a LDC Analytical-III system.
Pharmaspec 1700 uv visible spectrophotometer
Manufactured by Shimadzu
The Pharmaspec-1700 UV-Visible spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light by a sample. It operates in the ultraviolet and visible light wavelength ranges. The Pharmaspec-1700 can be used to quantify the concentration of a substance in a solution by comparing the light absorption of the sample to a known standard.
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Lab products found in correlation
2 protocols using pharmaspec 1700 uv visible spectrophotometer
1
Spectroscopic Characterization of Compounds
2
Solubility Enhancement of Berberine Nanoparticles
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Solubility is the maximum amount of a compound/material in a solution for a particular solvent. The solubility of large particles (micrometers or more) is generally not dependent on particle size. However, the solubility of NPs mainly depends on particle size, with an increase of solubility as the particle size decreases.34 (link)
For the solubility study, a surplus amount (equivalent to 200 mg) of unprocessed BBR and the prepared NPs were placed in separate vials. Ten mL of distilled water was added to each vial and shaken vigorously in an orbital shaker (HS501 orbital shaker, IKA GmbH, Staufen im Breisgau, Germany) at 25°C (room temperature) for 72 hours at 3,000 rpm. After mixing, samples were centrifuged at 3,000 rpm and filtered through a Whatman filter paper no 1 (Thermo Fisher Scientific, Waltham, MA, USA). For determination of solubility, the filtered portion was diluted and analyzed at 263 nm using a UV-visible spectrophotometer (PharmaSpec 1,700 UV-visible spectrophotometer, Shimadzu). The same procedure was adopted for PBS pH 6.8 and 0.1 M HCl as solvents to determine the solubility. All tests were run in triplicate. The data were evaluated to determine their significance by applying statistical analysis (one-way ANOVA followed by Dunnett’s post hoc test).
For the solubility study, a surplus amount (equivalent to 200 mg) of unprocessed BBR and the prepared NPs were placed in separate vials. Ten mL of distilled water was added to each vial and shaken vigorously in an orbital shaker (HS501 orbital shaker, IKA GmbH, Staufen im Breisgau, Germany) at 25°C (room temperature) for 72 hours at 3,000 rpm. After mixing, samples were centrifuged at 3,000 rpm and filtered through a Whatman filter paper no 1 (Thermo Fisher Scientific, Waltham, MA, USA). For determination of solubility, the filtered portion was diluted and analyzed at 263 nm using a UV-visible spectrophotometer (PharmaSpec 1,700 UV-visible spectrophotometer, Shimadzu). The same procedure was adopted for PBS pH 6.8 and 0.1 M HCl as solvents to determine the solubility. All tests were run in triplicate. The data were evaluated to determine their significance by applying statistical analysis (one-way ANOVA followed by Dunnett’s post hoc test).
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