Whole blood samples were drawn from 21 patients (age 69±12) with DFUs but free of active infection and existing systemic coagulation disorders. PPP was isolated from the blood specimens with the first centrifugation in the department of blood transfusion. Platelets were sequestered by full automatic blood separator (CS-3000 plus; Baxter International, Inc., Deerfield, IL, USA), and condensed by the second centrifugation to produce PRP. The concentrations of platelets in PRP and PPP were determined by the automatic hematology analyzer (KX-21N; Sysmex Corporation, Kobe, Japan). The average platelet count was (1024±111)×109/L in PRP, while it was <10×109/L in PPP. The majority of the extracted PRP was used to treat the blood donor’s DFUs while the remaining PRP will be used in our study. The above processes were approved by the institutional review board of the first hospital affiliated to the Army Medical University. Written informed consent was obtained from all patients. The study was approved by the medical ethics committee of the first hospital affiliated to the Army Medical University (Chongqing, People’s Republic of China) and conducted in compliance with the Declaration of Helsinki.
Cs 3000 plus
Manufactured by Baxter
Sourced in United States
The CS-3000 plus is a laboratory centrifuge designed for general-purpose applications. The device features programmable operation and digital controls for setting parameters such as speed, time, and temperature. The core function of the CS-3000 plus is to separate components of a liquid mixture based on their density differences by applying centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cs 3000 plus
1
Platelet-Rich Plasma for Diabetic Foot Ulcers
2
PBMC Apheresis for Cell Separation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PBMC aphereses were performed as previously described,10 using either a Baxter‐Fenwal CS‐3000 Plus (1 dog) or TerumoBCT (formally CaridianBCT) (14 dogs) cell separator machine. In 4 dogs weighing <15 kg, the TerumoBCT cell separator was primed to ensure hemodynamic stability during the initial period of apheresis. Three of these dogs have been previously described.11 The priming solution used in the remaining dog (6.7 kg) was 100 mL autologous whole blood and 100 mL 0.9% NaCl. In addition, the rinse back products (purged machine contents after completion of apheresis) in these cases were collected into an empty bag rather than being returned to the dogs. This blood (approximately 400 mL) was subsequently infused into the dogs at approximately 50 mL/h beginning immediately after the aphereses were completed.
3
Anticoagulant-Preserved Platelet-Rich Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood (400 mL) was gained from three healthy adults recruited from the
Department of Blood Transfusion, Southwest Hospital, Army Medical University,
Chongqing, China. Volunteers did not take any drugs that would have affected
platelet number or function within the 2 weeks before blood donation. All
volunteers provided written informed consent following the approval of the
Ethical Committee Board of Southwest Hospital. Citrate glucose (22 g/L sodium
citrate, 24.5 g/L glucose monohydrate, and 8 g/L citric acid monohydrate) was
used as an anticoagulant for blood samples at a ratio of 1:10, and 100 ng/mL of
prostaglandin E1 (PGE1, Sigma-Aldrich, St. Louis, MO, USA) was added to prevent
platelet activation during isolation. PRP (40 mL) was isolated by a
fully-automatic blood separator (CS-3000 plus; Baxter International, Inc.,
Deerfield, IL, USA), which has a leukocyte reduction system to avoid leukocyte
contamination. The concentration of apheresis platelets in PRP and venous blood
were counted by the automatic hematology analyzer (KX-21 N; Sysmex Corporation,
Kobe, Japan). The prepared PRP was stored at 24°C while shaking or stored at
-80°C until ready to use.
Department of Blood Transfusion, Southwest Hospital, Army Medical University,
Chongqing, China. Volunteers did not take any drugs that would have affected
platelet number or function within the 2 weeks before blood donation. All
volunteers provided written informed consent following the approval of the
Ethical Committee Board of Southwest Hospital. Citrate glucose (22 g/L sodium
citrate, 24.5 g/L glucose monohydrate, and 8 g/L citric acid monohydrate) was
used as an anticoagulant for blood samples at a ratio of 1:10, and 100 ng/mL of
prostaglandin E1 (PGE1, Sigma-Aldrich, St. Louis, MO, USA) was added to prevent
platelet activation during isolation. PRP (40 mL) was isolated by a
fully-automatic blood separator (CS-3000 plus; Baxter International, Inc.,
Deerfield, IL, USA), which has a leukocyte reduction system to avoid leukocyte
contamination. The concentration of apheresis platelets in PRP and venous blood
were counted by the automatic hematology analyzer (KX-21 N; Sysmex Corporation,
Kobe, Japan). The prepared PRP was stored at 24°C while shaking or stored at
-80°C until ready to use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!