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Anti nur77

Manufactured by Cell Signaling Technology
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Anti-Nur77 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Nur77 protein, which is a transcription factor involved in various cellular processes. The primary function of Anti-Nur77 is to detect and analyze the expression and localization of Nur77 in biological samples.

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Lab products found in correlation

Anti parp, by Santa Cruz Biotechnology (4 mentions) Jc 1 probe, by Thermo Fisher Scientific (3 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (3 mentions) Anti pcna, by Santa Cruz Biotechnology (3 mentions) Anti bcl 2, by Santa Cruz Biotechnology (3 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti α tubulin, by Santa Cruz Biotechnology (2 mentions) Anti bcl 2 antibody, by Abmart (2 mentions) Fluorescent probes of mito tracker deep red, by Thermo Fisher Scientific (2 mentions) Anti myc 9e10, by Santa Cruz Biotechnology (2 mentions) Anti flag, by Merck Group (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti nur77 m 210, by Santa Cruz Biotechnology (1 mentions) Mitotracker deep red, by Merck Group (1 mentions) Anti hsp60, by Abcam (1 mentions) Anti c myc sc 40, by Santa Cruz Biotechnology (1 mentions) Anti dicer, by Cell Signaling Technology (1 mentions) Mk2206, by Beyotime (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti β actin 66009 1 ig, by Proteintech (1 mentions)

6 protocols using anti nur77

1

Multiparametric Evaluation of Mitochondrial Function

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Anti-Myc (9E10) (Cat. Sc-40), anti-Ki67 (Cat. ab15580) and anti-Hsp60 (Cat. ab46798) antibodies were purchased from Abcam (UK); anti-β-actin (Cat. 4970S), anti-Cleaved caspase-3 (Cat. 9661S), and anti-Nur77 (Cat. 3960S) antibodies were purchased from Cell Signal Technology (Beverly, MA, USA); anti-Nur77 (M-210) (Cat. sc-5569), anti-PCNA (Santa Cruz sc-7907), anti-a-tublin (Santa Cruz sc-8035), anti-Bcl-2 (Santa Cruz sc-783), anti-PARP (Santa Cruz sc-7150), and anti-GST (sc-138) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-Flag (Cat.F1804) antibody was purchased from Sigma (St. Louis, MO, USA); Mito-tracker deep red (Cat. M22426), JC-1 Probe (Cat. T3168) and mitoSOX Red Mitochondrial Superoxide Indicator (Cat. M36008) were purchased from Thermo Fisher.
Chen X., Cao X., Tu X., Alitongbieke G., Xia Z., Li X., Chen Z., Yin M., Xu D., Guo S., Li Z., Chen L., Zhang X., Xu D., Gao M., Liu J., Zeng Z., Zhou H., Su Y, & Zhang X.K. (2019). BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway. Molecular cancer therapeutics, 18(5), 886-899.
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2

Antibody Validation for Western Blotting

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For Western blotting, antibodies of anti-Ki67 (Abcam plc, ab15580), anti-Hsp60 (Abcam plc, ab46798), anti-β-actin (Cell Signaling Technology, 4970S), anti-cleaved caspase-3 (Cell Signaling Technology, 9661S), anti-Nur77 (Cell Signaling Technology, 3960S), anti-Myc (9E10) (Santa Cruz Biotechnology, Sc-40), anti-PCNA (Santa Cruz Biotechnology, sc-7907), anti-α-Tubulin (Santa Cruz Biotechnology, sc-8035), anti-Bcl-2 (Santa Cruz Biotechnology, sc-783), anti-Bcl-2 antibody (Abmart, T40056F), anti-PARP (Santa Cruz Biotechnology, sc-7150) were purchased and all the above antibodies were used in the dilution ratio of 1:1000. For immunoprecipitation, anti-Flag (Sigma–Aldrich, F1804, dilution: 1:100), anti-Bcl-2 (Santa Cruz Biotechnology, sc-65392, dilution: 1:50) was utilized. For immunofluorescence staining, Fluorescent Probes of Mito-tracker deep red (ThermoFisher Scientific, M22426, dilution: 1:20,000), JC-1 Probe (Thermo Fisher Scientific, T3168, 1:500) and Mito-SOX Red Mitochondrial Superoxide Indicator (Thermo Fisher Scientific, M36008, dilution: 1:3000) were utilized.
Chen X., Gao M., Xia Y., Wang X., Qin J., He H., Liu W., Zhang X., Peng S., Zeng Z., Su Y, & Zhang X. (2023). Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 condensates. Acta Pharmaceutica Sinica. B, 14(3), 1204-1221.
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3

Antibody Validation for Western Blotting

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For Western blotting, antibodies of anti-Ki67 (Abcam plc, ab15580), anti-Hsp60 (Abcam plc, ab46798), anti-β-actin (Cell Signaling Technology, 4970S), anti-cleaved caspase-3 (Cell Signaling Technology, 9661S), anti-Nur77 (Cell Signaling Technology, 3960S), anti-Myc (9E10) (Santa Cruz Biotechnology, Sc-40), anti-PCNA (Santa Cruz Biotechnology, sc-7907), anti-α-Tubulin (Santa Cruz Biotechnology, sc-8035), anti-Bcl-2 (Santa Cruz Biotechnology, sc-783), anti-Bcl-2 antibody (Abmart, T40056F), anti-PARP (Santa Cruz Biotechnology, sc-7150) were purchased and all the above antibodies were used in the dilution ratio of 1:1000. For immunoprecipitation, anti-Flag (Sigma–Aldrich, F1804, dilution: 1:100), anti-Bcl-2 (Santa Cruz Biotechnology, sc-65392, dilution: 1:50) was utilized. For immunofluorescence staining, Fluorescent Probes of Mito-tracker deep red (ThermoFisher Scientific, M22426, dilution: 1:20,000), JC-1 Probe (Thermo Fisher Scientific, T3168, 1:500) and Mito-SOX Red Mitochondrial Superoxide Indicator (Thermo Fisher Scientific, M36008, dilution: 1:3000) were utilized.
Chen X., Gao M., Xia Y., Wang X., Qin J., He H., Liu W., Zhang X., Peng S., Zeng Z., Su Y, & Zhang X. (2023). Phase separation of Nur77 mediates XS561-induced apoptosis by promoting the formation of Nur77/Bcl-2 condensates. Acta Pharmaceutica Sinica. B, 14(3), 1204-1221.
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4

Molecular Profiling of Stem Cell Markers

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Anti-Nur77 (#3960), anti-Dicer (#5362), and anti-phospho-Akt (#4060) were purchased from Cell Signaling Technology (Danvers, MA, USA); anti-phospho-serine/threonine (ab15556) from Abcam (Cambridge, MA, USA); anti-p63 (12143-1-AP), anti-CD44 (15675-1-AP), anti-CD133 (18470-1-AP), anti-EpCAM (21050-1-AP), anti-ALDH-1 (15910-1-AP), and anti-β-actin (66009-1-Ig) from Proteintech (Rosemont, Inc., Rosemont, CA, USA); anti-total Akt (sc-81434), anti-p110α (sc-293172), anti-GFP (sc-9996), anti-Flag (sc-166355) and anti-c-Myc (sc-40) from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Lipofectamine 2000 from Invitrogen (Carlsbad, CA, USA); Compound K from AMCEC (Shanghai, China); MK-2206 from Beyotime (Shanghai, China); and fetal bovine serum (FBS) from Gibco (Grand Island, NY, USA).
Zhang M., Shi Z., Zhang S., Li X., To S.K., Peng Y., Liu J., Chen S., Hu H., Wong A.S, & Zeng J.Z. (2022). The Ginsenoside Compound K Suppresses Stem-Cell-like Properties and Colorectal Cancer Metastasis by Targeting Hypoxia-Driven Nur77-Akt Feed-Forward Signaling. Cancers, 15(1), 24.
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5

Antibody-based Protein Detection Assay

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The antibodies used in this study were anti-β-actin (#66009–1-Ig, Proteintech), anti-LC3 (#14600–1-AP, Proteintech), anti-Cleaved PARP (#A19612, Abclonal), anti-α-tubulin (#2125, Cell Signalling Technology), anti-Lamin B1 (#A11495, Abclonal), anti-Nur77 (#3960, Cell Signalling Technology), and anti-PARP (#SC-8007, Santa Cruz Biotechnology).
He F., Chen J., Zhao T., Wu Q., Yin N., Wang X., Zhong Y., Guo X., Qiu Y., Li B., Fang M, & Wu Z. (2023). Design, synthesis, and evaluation of novel benzoylhydrazone derivatives as Nur77 modulators with potent antitumor activity against hepatocellular carcinoma. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2227777.
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6

Inflammasome Protein Quantification

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The cells were lysed with lysis buffer, separated by SDS-PAGE, and transferred to polyvinylidene difluoride membrane. Membranes then were blocked with 5% nonfat milk and incubated with primary antibody, followed by detection using an enhanced chemiluminescence system. The following primary antibodies were used: anti-NLRP3 (Cat# ab214185, Abcam), anti-caspase-1 (Cat# 3866, Cell Signaling Technology), anti-cleaved caspase-1 (Cat# 3866, Cell Signaling Technology), anti-IL-1β (Cat# AB41610, Absci), anti-cleaved IL-1β (Cat# AB41610, Absci), anti-GSMD (Cat# 39754, Cell Signaling Technology), anti-cleaved GSMD (Cat# 39754, Cell Signaling Technology), and anti-Nur77 (Cat# 3960, Cell Signaling Technology).
Yuan R., Zhang W., Nie P., Lan K., Yang X., Yin A., Xiao Q., Shen Y., Xu K., Wang X., Pan X., Shen L, & He B. (2022). Nur77 Deficiency Exacerbates Macrophage NLRP3 Inflammasome-Mediated Inflammation and Accelerates Atherosclerosis. Oxidative Medicine and Cellular Longevity, 2022, 2017815.
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