Pyragallol

All reagents used in this study were of analytical grade. 5,5’-Dithiobis-2-Nitrobenzoic Acid (DTNB), 1-chloro-2,4-dinitrobenzene (CDNB), Thiobarbituric acid and Nicotinamide Adenine Dinucleotide Phosphate Dehydrogenase (NADPH) were purchased from Sisco Research Laboratories (India). 2,4,6-Tri-(2-Pyridyl)-5-Triazine (TPTZ), Pyragallol, Reduced Glutathione, Oxidized Glutathione, 2,4-Dinitrophenyl Hydrazine (DNPH), Tri-fluoroacetic Acid and Guanidine Hydrochloride were obtained from Sigma (USA). N-(1-Naphthyl) ethylene-diaminedihydrochloride (NED), Hydrogen Peroxide and Sulphanilamide were obtained from Merck (USA). PureLink@ RNA mini kit (Invitrogen), High Capacity cDNA Reverse Transcription Kit (Applied Biosystems), Primers for IFN-γ, TNF-α and β-actin procured and synthesized from Eurofins Genomics, India Pvt. Ltd., while ELISA cytokine detection kits procured from R&D Systems, Minneaopolis, USA. Highly purified distilled water was obtained from Millipore.

2,4,6-Tri-(2-Pyridyl)-5-Triazine (TPTZ), 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), 2′,7′-Dichlorofluorescein diacetate (DCF-DA), Reduced Glutathione, Oxidized Glutathione, Pyragallol, 2,4-Dinitrophenyl Hydrazine (DNPH), RPMI-1640 medium, Trifluoroacetic Acid and Thiobarbituric Acid were obtained from Sigma Aldrich (USA). 5,5’-Dithiobis(2-Nitrobenzoic Acid (DTNB), 1-chloro-2,4-dinitrobenzene (CDNB), ethidium bromide, agarose, Triton X-100 and Nicotinamide Adenine Dinucleotide Phosphate Reduced Tetrasodium Salt (NADPH) were purchased from Sisco Research Laboratories (New Delhi, India). Sulfanilamide and Guanidine Hydrochloride were purchased from Bio Basic Inc. (Ontario, Canada). N-(1-Naphthyl) ethylene diamine dihydrochloride (NED) and Hydrogen Peroxide were obtained from Merck (USA). All other chemicals used in the study were of analytical grade.

Methanol, Hydrochloric acid (HCL), Sulfuric acid (H₂SO₄), Ethanol, Petroleum ether, Triton-X, Sodium carbonate (Na₂CO₃), Sodium Hydroxide (NaOH), Copper sulphate (CuSO₄), Potassium sodium tartarate (KNaC₄H₄O₆.4H₂O), Bovine serum albumin (BSA), Gallic acid, Piperine, quercetin, Aluminum nitrate (Al (NO₃)₃), 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, Aluminum chloride (AlCl₃), potassium acetate, Trichloroacetic acid (TCA), 5,5’-dithio-bis(2-nitrobenzoic acid) (DTNB), Hydrogen Peroxide (H₂O₂), potassium dihydrogen phosphate, Sodium Tartarate, pyragallol, potassium hydroxide, and di-potassium hydrogen phosphate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ketamine was obtained from Caraway pharmaceutical (Islamabad, Pakistan). Galantamine was obtained from Reko Pharmacia (Lahore, Pakistan). All the chemicals used were of analytical grade.