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Caspase 3 activity

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Caspase 3 is an enzyme involved in the process of apoptosis, or programmed cell death. It plays a crucial role in the execution phase of cell apoptosis. The Caspase 3 Activity Assay is a quantitative colorimetric or fluorometric assay used to measure the activity of Caspase 3 in cell or tissue lysates.

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Lab products found in correlation

Ifn λ, by PBL Assay Science (1 mentions) Ifn α, by PBL Assay Science (1 mentions) Ifn β, by R&D Systems (1 mentions)

Close spelling variants of this product

Caspase 3/7 activity assay (9 citations) Caspase 3/7 activity (8 citations) Caspase 3/7 activity assay kit (8 citations) Caspase-3 (7 citations) Caspase-Glo 3/7 activity assay (7 citations) Caspase-3 activity assay kit (7 citations) Caspase-Glo 3/7 Activity kit (6 citations) Caspase-Glo® 3/7 Activity assay kit (6 citations) Apo-ONE homogenous caspase 3/7 activity assay kit (5 citations) Apo-ONE homogeneous caspase-3 activity kit (2 citations)

2 protocols using caspase 3 activity

1

Caspase 3 Activity Assay in Suspended Cells

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We collected cells from suspended culture media and analysed caspase 3 activity according to manufacture's instructions (Promega, Madison, WI, USA).
Kim Y.H., Choi Y.W., Lee J., Soh E.Y., Kim J.H, & Park T.J. (2017). Senescent tumor cells lead the collective invasion in thyroid cancer. Nature Communications, 8, 15208.
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2

Interferon-Induced Apoptosis in Cells

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Human interferons used were commercially available recombinant proteins i.e. IFN-α (PBL Assay Science, Piscataway, NJ, USA), IFN-β (R&D System, MN, USA) and IFN-λ (PBL). Confluent normal and tumor cell lines were prepared in a 12-well plates. Cells were washed and medium was replaced with fresh maintenance medium supplemented with IFN-α, -β and -γ (1000 IU each) or IFN-α, -β (1500 IU each) or IFN-γ (3000 IU) in 1 mL medium. Plates were then incubated for 24 hours at 37 °C and cell lysate was subjected to caspase-3 activity (Promega).
Ginting T.E., Christian S., Larasati Y.O., Suryatenggara J., Suriapranata I.M, & Mathew G. (2019). Antiviral interferons induced by Newcastle disease virus (NDV) drive a tumor-selective apoptosis. Scientific Reports, 9, 15160.
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