Immuno-staining and image analysis were performed as described in McKim et al. (2009) (link) and Zhang et al. (2012a) (link). In short, 2–4 days old female ovaries were dissected in Robb’s buffer, fixed with 4% formaldehyde, washed, overnight incubated with primary antibody, washed, incubated overnight with secondary antibody with the fluorophore, stained with DAPI for DNA labeling and mounted on slide with mounting medium. To enhance the GFP signal, ChromoTek anti-GFP Booster (Atto-488) antibody was added with secondary antibody. Antibodies used: anti-GFP Booster (ChromoTek) at 1:200, guinea pig anti-Rhi (our lab) at 1:1000, rabbit anti-Del (from Julius Brennecke) at 1:1000, rabbit anti-TRF2 (from James Kadonaga) at 1:500, rabbit anti-H3K9me3 (abcam) at 1:1000.
Rabbit anti trf2
Manufactured by Abcam
Rabbit anti-TRF2 is a primary antibody that specifically recognizes the TRF2 (Telomeric Repeat-Binding Factor 2) protein. TRF2 is a key component of the shelterin complex, which is responsible for maintaining the structural integrity and regulation of telomeres, the protective caps at the ends of chromosomes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti trf2
1
Immuno-staining and Imaging of Drosophila Ovaries
2
Immuno-staining and Imaging of Drosophila Ovaries
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Immuno-staining and image analysis were performed as described in McKim et al. (2009) (link) and Zhang et al. (2012a) (link). In short, 2–4 days old female ovaries were dissected in Robb’s buffer, fixed with 4% formaldehyde, washed, overnight incubated with primary antibody, washed, incubated overnight with secondary antibody with the fluorophore, stained with DAPI for DNA labeling and mounted on slide with mounting medium. To enhance the GFP signal, ChromoTek anti-GFP Booster (Atto-488) antibody was added with secondary antibody. Antibodies used: anti-GFP Booster (ChromoTek) at 1:200, guinea pig anti-Rhi (our lab) at 1:1000, rabbit anti-Del (from Julius Brennecke) at 1:1000, rabbit anti-TRF2 (from James Kadonaga) at 1:500, rabbit anti-H3K9me3 (abcam) at 1:1000.
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