Irdye800 conjugated goat anti mouse

Manufactured by LI COR

The IRDye800-conjugated goat anti-mouse is a secondary antibody labeled with the IRDye800 fluorescent dye. It is designed for use in fluorescence-based detection and imaging applications.

Automatically generated - may contain errors

Lab products found in correlation

Odyssey blocking buffer, by LI COR (2 mentions) Pvdf fl membrane, by Merck Group (2 mentions) Odyssey imager, by LI COR (1 mentions) Ab186407, by Abcam (1 mentions) Ab6556, by Abcam (1 mentions) Alpha tubulin, by Abcam (1 mentions) Image studio 3, by LI COR (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Irdye 680 conjugated goat anti rabbit, by LI COR (1 mentions)

Spelling variants of this product

IRdye800-conjugated goat anti-mouse IgG secondary antibody (3 citations) IRDye800-conjugated goat anti-rabbit and anti-mouse IgG (2 citations) Polyclonal goat IRdye800-conjugated anti-mouse IgG (2 citations)

2 protocols using irdye800 conjugated goat anti mouse

Western Blot Analysis of DPD Protein

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Protein lysates were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF)-FL membrane (EMD Millipore, Saint Charles, MO). Membranes were blocked using Odyssey blocking buffer (LI-COR Biosciences, Lincoln, NE). Blots were probed with primary antibodies against DPD and α-tubulin (ab6556 and ab186407, respectively; Abcam, Cambridge, MA). IRDye800-conjugated goat anti-mouse and IRDye680-conjugated goat anti-rabbit secondary antibodies (LI-COR) were used for detection. Blots were scanned on a LI-COR Odyssey imager.

Nie Q., Shrestha S., Tapper E.E., Trogstad-Isaacson C.S., Bouchonville K.J., Lee A.M., Wu R., Jerde C.R., Wang Z., Kubica P.A., Offer S.M, & Diasio R.B. (2017). Quantitative contribution of rs75017182 to dihydropyrimidine dehydrogenase mRNA splicing and enzyme activity. Clinical pharmacology and therapeutics, 102(4), 662-670.

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Western Blot Protein Detection

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Protein lysates were separated on a 10% SDS-PAGE gel and transferred to PVDF-FL membrane (EMD Millipore). Membranes were blocked using Odyssey blocking buffer (LI-COR, Lincoln, NE). Blots were probed with primary antibodies against DPD and alpha-tubulin (both AbCam, Cambridge, MA) and subsequent secondary IRDye800 conjugated goat anti-mouse and IRDye 680 conjugated goat anti-rabbit (both LI-COR). Blots were scanned and band intensities quantified using the Image Studio 3.1 and LI-COR Odyssey Infrared Imaging system according to manufacturer’s instructions.

Offer S.M., Butterfield G.L., Jerde C.R., Fossum C.C., Wegner N.J, & Diasio R.B. (2014). MicroRNAs miR-27a and miR-27b directly regulate liver dihydropyrimidine dehydrogenase expression through two conserved binding sites. Molecular cancer therapeutics, 13(3), 742-751.

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