Meninflox flox mice

Menin^flox/flox mice, TCR-βδ KO mice and Cre TG mice under the control of the Cd4 promoter were purchased from The Jackson Laboratory. Menin^flox/flox × CD4 Cre Tg mice with BALB/c mice were generated by Dr Toshinori Nakayama (Chiba University, Chiba, Japan). Bach2^flox/flox mice were established by Dr Tomohiro Kurosaki (RIKEN and Osaka University)28 (link), OVA-specific αβTCR (DO11.10) TG mice were provided by Dr D. Loh (Washington University School of Medicine, St Louis, USA)54 (link), and C57BL/6 and BALB/c mice were purchased from Clea (Clea Japan, Tokyo, Japan). The female mice were used in in vivo experiments. For in vitro experiments, both male and female mice were used. All mice were maintained under specific pathogen-free conditions and were then used at 6-10 weeks of age. All experiments using mice received approval from the Kazusa DNA Research Institute and Ehime University Administrative Panel for Animal Care. All animal care was conducted in accordance with the guidelines of the Kazusa DNA Research Institute and Ehime University.

C57BL6 mice were purchased from Clea Japan, Inc. C57BL6 mice of 6–10 weeks of age and ≥30 weeks of age were used as young mice and old mice, respectively. Menin^flox/flox mice and Cre transgenic (Tg) mice under the control of the Cd4 promoter were purchased from The Jackson Laboratory. In addition, we generated WT OT‐1 Tg mice or Menin KO OT‐1 Tg mice by crossing WT mice or Menin KO mice with OT‐1 Tg mice. WT and Menin KO mice of 10–16 weeks of age were used in in vivo experiments. All the animal experiments received approval from the Ehime University Administrative Panel for Animal Care.