Sch B was purchased from Selleck Chemicals (Houston, TX, USA). Carbon tetrachloride (CCl4) was purchased from Chinasun Specialty Products Co., Ltd. (Changshu, Jiangsu, China). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-PPARγ, anti-F4/80, anti-alpha smooth muscle actin (α-SMA), anti-CD86, Lamin B1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were obtained from Affinity Biosciences (Changzhou, Jiangsu, China). Rabbit antibodies against nuclear factor (NF)-κB and phospho-IκBα were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies used in western blotting and immunohistochemical staining were purchased from Affinity Biosciences. Secondary antibodies used in immunofluorescence staining were purchased from Abcam (Cambridge, MA, USA).
Anti f4 80
Manufactured by Affinity Biosciences
Sourced in China, United States
The Anti-F4/80 is a laboratory reagent used for the detection and analysis of the F4/80 antigen, which is a glycoprotein expressed on the surface of macrophages in mice. This product can be utilized in various immunological techniques, such as flow cytometry, to identify and characterize macrophage populations in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd86, by Affinity Biosciences (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Affinity Biosciences (1 mentions)
Rabbit anti pparγ, by Affinity Biosciences (1 mentions)
Lamin b1, by Affinity Biosciences (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Anti tnf α, by Affinity Biosciences (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Agilent Technologies (1 mentions)
2 protocols using anti f4 80
1
Carbon Tetrachloride-Induced Liver Injury
2
Immunohistochemical Analysis of Renal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Renal tissue section (4 μm) were fixed in 4% formaldehyde. After deparaffinization and rehydration, IHC was performed on the sections using anti-TNF-α, anti-F4-80 (mouse EGF-like module-containing mucin-like hormone receptor-like 1), anti-4-HNE (4-Hydroxynonenal), anti-3-NT (3-nitrotyrosine), or anti-GPX4 primary antibody (all from Affinity Biosciences, OH, USA; 1:200). The primary antibodies were incubated at 4 °C overnight, followed by subsequent incubation with horseradish peroxidase-conjugated secondary antibody (Dako, Glostrup, Denmark) at a 1:200 dilution at room temperature (RT) for 1 h. The sections were washed with PBS following each process. In the absence of the primary antibody or upon the addition of excessive antigen, the staining condition was assessed to ensure specificity. The fluorescence image was captured by a fluorescence microscope (BX53, Olympus, × 400). Staining intensities were quantified with ImageJ software.
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