Emem cell culture media

Androgen-independent prostate cancer cell lines DU145 (ATCC #HTB-81) and PC3 (ATCC #CRL-1435), androgen-dependent prostate cancer cell line LNCaP (ATCC #CRL-1740), prostate-derived fibroblasts WPMY-1 (ATCC #CRL-2854) and hTERT PF179T (ATCC #CRL-3290) were purchased from American Type Culture Collection (Manassas, VA, USA). DU145 cells were maintained in Eagle’s Minimum Essential Medium (EMEM) cell culture media (Corning, Corning, NY, USA). PC3 cells were cultured in F12 cell culture media (Gibco, ThermoFisher, Waltham, MA, USA). LNCaP cells were cultured in RPMI-1640 culture media (ATCC 30–2001). WPMY-1 normal fibroblast cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM). Media was supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) Pen Strep (both purchased from Gibco) under humidified conditions at 37°C and 5% CO₂. hTERT PF179T cancer-associated fibroblast cells were maintained in EMEM supplemented with 10% (v/v) FBS, 1% (v/v) sodium bicarbonate 7.5% solution (#SH30033.01, GE Life Sciences Hyclone), and 0.01% 10 mg/mL puromycin (#A11138-03, Gibco).