The fixed maxillae were subjected to µCT scanning (Skyscan1275; Bruker Corporation) using a voxel size of 20 µm3 and a 0.5 mm aluminum filter at 55 kVp and 145 µA. Two-dimensional slices from each maxilla were combined using NRecon and CTAn/CTVol programs (Bruker Corporation) to form a three-dimensional reconstruction. Alveolar bone loss was quantified by measuring the distance from the palatal and mesiobuccal CEJ to the alveolar bone crest (ABC) of the second molars by an investigator (JS). The reading was confirmed in a double blind manner by another individual (N-HP).
Ctan ctvol
Manufactured by Bruker
Sourced in Belgium
The CTAn/CTVol is a software package for the analysis and visualization of 3D data from Computed Tomography (CT) imaging. It provides tools for quantitative analysis of morphometric parameters and 3D visualization of the scanned samples.
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Lab products found in correlation
4 protocols using ctan ctvol
1
Alveolar Bone Loss Quantification via µCT
2
Maxillary Bone Resorption Analysis
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The fixed maxillae were subjected to μCT scanning (Skyscan1275, Bruker-microCT, Kontich, Belgium) using a voxel size of 20 μm3 and a 0.5 mm aluminum filter. Two-dimensional slices from each maxilla were combined using NRecon and CTAn/CTVol programs (Bruker) to form a three-dimensional reconstruction. The level of bone resorption was calculated as the distance from the palatal and mesiobuccal CEJ to the ABC by the first author (J.S.S.). The reading was confirmed in a blinded-manner by another author (R.H.K.).
3
Micro-CT Analysis of Alveolar Bone
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The fixed maxillae were subjected to Micro-CT scanning (Skyscan1275, Bruker-microCT, Kontich, Belgium) using a voxel size of 20 μm3 and a 0.5 mm aluminium filter at 55 kVp and 145 μA. To form a three-dimensional reconstruction, two-dimensional slices from each maxilla were combined using NRecon and CTAn/CTVol programs (Bruker, Kontich, Belgium). Bone loss was quantified by measuring the distance from the palatal and mesiobuccal cement-enamel junction (CEJ) to the alveolar bone crest (ABC). Bone mineral density (BMD) was calculated based on the thresholds within the raw data obtained from Micro-CT and the change in the direction of the coronal section. The region of interest was preset with the help of the interpolation method of the alveolar bone. Additionally, each sample was analyzed for trabecular thickness (Tb.Th) and bone volume to tissue volume (BV/TV).
4
Quantifying Maxillary Bone Loss via μCT
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The fixed maxillae were subjected to μCT scanning (Skyscan1275, Bruker-microCT, Kontich, Belgium) using a voxel size of 20 μm3 and a 0.5 mm aluminum filter at 55 kVp and 145 μA. Two-dimensional slices from each maxilla were combined using NRecon and CTAn/CTVol programs (Bruker) to form a three-dimensional reconstruction. Bone loss was quantified by measuring the distance from the palatal and mesiobuccal cement-enamel junction (CEJ) to the alveolar bone crest (ABC).
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