Cell migration was assayed in 24‐well Transwell chambers with 8‐μm pore size filter membrane (Corning, Shanghai, China). On reaching 90% confluence in plates, cells were treated with serum‐free medium for 12 h. After removing the medium and floating cells, the cells were washed three times with sterile 1× phosphate‐buffered saline and then stained with hematoxylin–eosin. The number of cells that migrated to the bottom side of the insert was counted under a microscope.
Transwell chambers with 8 μm pore size filter membrane
Manufactured by Corning
Sourced in China
Transwell chambers with 8-μm pore size filter membrane provide a physical barrier to separate two compartments. The filter membrane allows the passage of small molecules and cells while retaining larger components.
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Lab products found in correlation
2 protocols using transwell chambers with 8 μm pore size filter membrane
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Transwell Cell Migration Assay
2
Cell Migration Assay Protocol
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Cell migration assay was performed using transwell chambers with 8 μm pore size filter membrane (Corning, Shanghai, China). HepG2 and Bel7404 were harvested and suspended with serum-free RPMI 1640 or DMEM, and then seeded into the upper chambers (1 × 105 cells/chambers), with the lower chambers containing 250 μl complete culture medium. After incubation 10 hours at 37 °C and 5% CO2 incubator, the inner side of the membranes were cleaned with wet cotton swabs to remove non-migrated cells. The outer sides of the membranes were washed with PBS twice, and then fixed with precooled methanol for 10 minutes and stained with crystal violet. The number of migrated cells for each filter membrane was calculated as the average of 5 random 300 μm × 300 μm fields. All of the experiments were performed three times to obtain robust results.
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