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7500 apparatus

Manufactured by Thermo Fisher Scientific
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Sourced in Japan

The 7500 apparatus is a laboratory equipment designed for performing a variety of analytical tasks. It features a compact and durable construction, making it suitable for use in various laboratory settings. The core function of the 7500 apparatus is to provide reliable and consistent results for the intended analytical procedures.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (3 mentions) Primescript rt reagent kit, by Takara Bio (3 mentions) Sybr green master mix, by Takara Bio (3 mentions) Multiskan fc, by MultiSciences Biotech (1 mentions) Jem 2010hr, by JEOL (1 mentions) Zetasizer nano zs analyzer, by Malvern Panalytical (1 mentions) Uv 2600 uv vis spectrophotometer, by PerkinElmer (1 mentions) Genomic mini purification kit, by A&A Biotechnology (1 mentions) Sybr green jumpstart taq readymix kit, by Merck Group (1 mentions)

Spelling variants of this product

ABI 7500 apparatus (17 citations) 7500 Sequence Detection System apparatus (7 citations) 7500 Real Time PCR System apparatus (4 citations) ABI Prism 7500 FAST apparatus (4 citations) ABI Prism 7500 apparatus (3 citations) 7500 Fast Real Time PCR System apparatus (3 citations) ABI Prism 7500 Real-Time PCR apparatus (3 citations) 7500 FAST apparatus (3 citations) Real-Time 7500 Fast apparatus (2 citations)

5 protocols using 7500 apparatus

1

Comprehensive Characterization of Nanoparticles

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The Supo HHS-6 electro-thermostatic water bath and the Evela N-1200A rotary evaporator were used. The freeze-drying method was operated by Biosafer Biosafer-10A and Christ Alpha 1–2 LD plus vacuum freeze-dryers. The size distribution and zeta potential were determined using a Malvern Zetasizer nano ZS analyzer. The UV used a Shimadzu UV-2600 UV-vis spectrophotometer, and the ATR-FTIR used a Perkin Elmer Spectrum two infrared analyzer. The JEOL JEM-2010HR was operated for TEM observation.
The cell cycles were performed by a Beckman CytoFLEX S flow cytometry analyzer. Cell proliferation was determined with a Multiskan FC ThermoFisher microplate reader. The quantitative RT-PCR was conducted with 7500 apparatus, Applied Biosystems (Waltham, MA, USA).
Yao M., Deng Y., Zhao Z., Yang D., Wan G, & Xu X. (2023). Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro. Molecules, 28(6), 2426.
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2

Transcriptome Analysis via qRT-PCR

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Total RNA was isolated using TRIzol reagent according to the manufacturer's instruction (Thermo Fisher, USA), and cDNA was synthesized by using the PrimeScript RT Reagent Kit (RR036A, Takara, Japan). The resulting cDNA was used for quantitative RT–PCR by using SYBR Green Master Mix (RR820B, Takara, Japan) in 7500 apparatus (Applied Biosystems). RT–PCR primer sequences are listed in Table EV1.
Lin Z., Niu Y., Wan A., Chen D., Liang H., Chen X., Sun L., Zhan S., Chen L., Cheng C., Zhang X., Bu X., He W, & Wan G. (2020). RNA m6A methylation regulates sorafenib resistance in liver cancer through FOXO3‐mediated autophagy. The EMBO Journal, 39(12), e103181.
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3

Total RNA Extraction and qRT-PCR Analysis

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Total RNA was isolated by using Trizol Reagent (Thermo Fisher, USA) from cells or patients' samples according to the manufacturer's instructions. The total RNA was reversely transcript into cDNA with the PrimeScript RT Reagent Kit (RR036A, Takara, Japan) and followed quantitative RT-PCR by using SYBR Green Master Mix (RR820A, Takara, Japan) in 7500 apparatus (Applied Biosystems, USA). The primers for RT-PCR were listed in Table S2.
Guan S., Chen X., Chen Y., Wan G., Su Q., Liang H., Yang Y., Fang W., Huang Y., Zhao H., Zhuang W., Liu S., Wang F., Feng W., Zhang X., Huang M., Wang X, & Zhang L. (2022). FOXO3 mutation predicting gefitinib-induced hepatotoxicity in NSCLC patients through regulation of autophagy. Acta Pharmaceutica Sinica. B, 12(9), 3639-3649.
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4

Plasmid Copy Number Quantification

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The copy number of pRK415 and its derivatives was measured by qPCR using the SYBR® Green JumpStart™ Taq ReadyMix kit (Sigma). The single-copy galK, gene from E. coli chromosome, was used as the chromosomal reference gene (primers #26 and #27) for all strains. The trfA gene of RK2 was used as the plasmid reference gene (primers #28 and #29) for pRK415 derivatives and pMPB9.90 (araBADp-trfARK2) whereas repB gene was amplified for plasmid RA3 (primers #30 and #31) (Table 2). Total DNA was purified from 4 ml of stationary-phase cultures using Genomic Mini purification kit (A&A Biotechnology), treated with an appropriate restriction enzyme to linearize the plasmid DNA and to fragment chromosomal DNA and then used as a template in qPCR. Plasmid copy number (PCN) was calculated relatively to the chromosomal marker on the basis of at least three biological replicates with three technical replicates per strain and average results with standard deviation are reported. The amplification, detection and analysis were carried out in the Laboratory of Genetic Modification Analyses of IBB PAS on an Applied Biosystems 7500 apparatus.
Bartosik A.A., Glabski K., Kulinska A., Lewicka E., Godziszewska J., Markowska A, & Jagura-Burdzy G. (2016). Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria. BMC Microbiology, 16, 59.
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5

Quantitative RT-PCR Analysis of RNA

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Total RNA was isolated using Trizol reagent according to the manufacturer’s instruction (ThermoFisher, USA) and the cDNA was synthesized by using the PrimeScript RT reagent Kit (RR036A, Takara, Japan). The resulting cDNA was used for quantitative RT-PCR by using SYBR-Green Master mix (RR820B, Takara, Japan) in 7500 apparatus (Applied Biosystems). RT-PCR primer sequences are listed in Table S1.
Lin Z., Wan A.H., Sun L., Liang H., Niu Y., Deng Y., Yan S., Wang Q.P., Bu X., Zhang X., Hu K., Wan G, & He W. (2022). N6-methyladenosine demethylase FTO enhances chemo-resistance in colorectal cancer through SIVA1-mediated apoptosis. Molecular Therapy, 31(2), 517-534.
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