After 24 h incubation in 30 °C, the cells were centrifuged and removed from the culture medium with saline. The inoculum suspension in saline with McFarland density of 0.5 was carefully spread on Petri plates with 4-mm thick MRS agar. When the suspension was absorbed by the agar, rings with antibiotics were distributed in triplicate. Erythromycin E15, kanamycin K30, bacitracin B10, streptomycin S10, amoxycillin AML25, tetracycline TE30, trimethoprim WE, penicillin P10, pirlimycin PIR2, chloramphenicol C30, and nalidixic acid NA30 were purchased from Oxoid (Hampshire, England).
Erythromycin e15
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Erythromycin (E15) is a macrolide antibiotic used in laboratory settings. It is a fermentation product derived from the bacterium Saccharopolyspora erythraea. Erythromycin (E15) inhibits bacterial protein synthesis by binding to the 50S subunit of the bacterial ribosome.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin s10, by Thermo Fisher Scientific (2 mentions)
Penicillin p10, by Thermo Fisher Scientific (2 mentions)
Chloramphenicol c30, by Thermo Fisher Scientific (2 mentions)
Nalidixic acid na30, by Thermo Fisher Scientific (2 mentions)
Penicillin g p10, by Thermo Fisher Scientific (2 mentions)
Vancomycin va30, by Thermo Fisher Scientific (2 mentions)
Silver nitrate agno3, by Merck Group (2 mentions)
Staphylococcus aureus atcc 6538, by American Type Culture Collection (1 mentions)
Pseudomonas aeruginosa atcc 10145, by American Type Culture Collection (1 mentions)
Levofloxacin lev 5, by Thermo Fisher Scientific (1 mentions)
Nitrofurantoin f300, by Thermo Fisher Scientific (1 mentions)
Ciprofloxacin cip 5, by Thermo Fisher Scientific (1 mentions)
S typhimurium, by American Type Culture Collection (1 mentions)
V parahaemolyticus atcc 17802, by American Type Culture Collection (1 mentions)
4 protocols using erythromycin e15
1
Antibiotic Susceptibility Assay
2
Antimicrobial Susceptibility Assay
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All media were bought from Difco, MB Cell (Seoul, Korea). Standard antibiotics discs of erythromycin (E15) 15 μg/disc, penicillin G (P10) 6 μg/disc and vancomycin (VA30) 30 μg/disc were purchased from Oxoid Ltd., England. Analytical grade AgNO3 (silver nitrate) was obtained from Sigma–Aldrich (St. Louis, MO, USA). The pathogenic bacterial strains Pseudomonas aeruginosa [ATCC 10145], Escherichia coli [ATCC 10798], and Staphylococcus aureus [ATCC 6538] were obtained from the American Type Culture Collection (ATCC).
3
Antimicrobial Susceptibility of Bacterial Pathogens
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The antimicrobial sensitivity test of all isolated Salmonella, E. coli, and Staphylococcus strains was conducted according to Koneman et al. [14 ] by the disc diffusion method, and different antibiotic discs were used such as ampicillin Amp10, chloramphenicol C30, ciprofloxacin CIP5, colistin sulfate CT25, danofloxacin DX, doxycycline Do30, enrofloxacin ENR5, erythromycin E15, fosfomycin FOS-200, levofloxacin LEV-5, nalidixic acid NA30, norfloxacin NOR10, nitrofurantoin F300, penicillin P10, streptomycin S10, tetracycline T30, and trimethoprim-sulfamethoxazole 25 SXT; Oxoid. The results were interpreted according to the Clinical and Laboratory Standards Institute [15 ].
4
Antibacterial Activity of Silver Nanoparticles
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All culture media were bought from Difco, MB Cell (Seoul, South Korea). AgNO3 (Silver nitrate) was bought from Sigma-Aldrich. Two pathogens V. parahaemolyticus (ATCC 17802) and S. Typhimurium (ATCC 14028) were collected from the American Type Culture Collection (ATCC). Standard antibiotics discs: Oleandomycin (OL15) 15 μg/disc, erythromycin (E15) 15 μg/disc, lincomycin (MY15) 15 μg/disc, vancomycin (VA30) 30 μg/disc, and penicillin G (P10) 10 μg/disc were gained from Oxoid Ltd., England.
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