Goat anti olig2 antibody

The following antibodies were used: mouse anti-HA antibody (1:500, BioLegend), rabbit anti-Giantin antibody (1: 1000, Biolegend), rabbit anti-PDI antibody (1:50, CST), goat anti-Olig2 antibody (1:200, R&D), rabbit anti-GFAP antibody (1:200, Proteintech), rabbit anti-IBA1 antibody (1:250, Invitrogen), mouse anti-Neu-N antibody (1:100, Millipore), rabbit anti-HCoV-OC43-N antibody, Cy3-conjugated donkey anti-rabbit antibody (1:500, Jackson), Alexa Fluor 488-conjugated donkey anti-mouse antibody (1:500, Jackson), Alexa Fluor 555-conjugated donkey anti-mouse antibody (1:500, Jackson), Alexa Fluor 488 anti-HA.11 Epitope Tag Antibody (1:500, Biolegend), Cy3-conjugated donkey anti-goat antibody (1:500, Jackson).
17CL-1 Cells infected with the virus (MOI = 0.1) were fixed using 4% PFA in PBS, then the cells permeabilized with 0.2% Triton X-100 in PBS. Cells were incubated with primary antibody at RT for 1 h, and then incubated with second antibody at RT for 1 h, the nucleus was stained with DAPI. After three times wash with PBS, the coverslips were mounted on glass slides. Cells were examined by confocal microscopy (ZEISS).

Brain cryosections were incubated with a primary mouse anti-ZIKV E protein antibody (Abcam) (1:500 dilution), goat anti-SOX2 antibody (R&D) (1:500 dilution), or goat anti-Olig2 antibody (R&D) (1:50 dilution) overnight at 4°C. The sections were then incubated with Alexa Fluor 488-conjugated anti-mouse and 594-conjugated anti-goat secondary antibodies (Thermo Fisher) (1:300 dilution) for 2 h at 37°C. Finally, the sections were incubated with DAPI (4′,6-diamidino-2-phenylindole) (1:1,000 dilution) for 10 min. Images were acquired by confocal laser scanning microscopy (Zeiss).