For pre-cultures of the bacteria the basic buffer recipe was 10g/L yeast extract (Becton Dickinson, Franklin Lakes, USA) and 10g/L soytone (Becton Dickinson, Franklin Lakes, USA). We refer to that buffer as 1xNutrient medium (also 1xNu). The initial pH was 7 and 100mM phosphate were added. For the washing steps and the experiments itself the medium contained 1g/L yeast extract and 1g/L soytone, 0.1mM CaCl2, 2mM MgCl2, 4mg/L NiSO4, 50mg/L MnCl2 and 1x Trace Element Mix (Teknova, Hollister, USA). We refer to that buffer as base buffer. It was supplemented with phosphate buffer and/or glucose as outlined in the single experiments. The usual concentration was 10g/L glucose, deviations from that are described for the single experiments below. All media were filter sterilized.
Trace element mix
Manufactured by Teknova
Sourced in United States
Trace Element Mix is a laboratory reagent that provides a blend of essential trace elements. It is a multi-component solution designed for use in various scientific applications that require a source of trace minerals.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using trace element mix
1
Bacterial Growth Media Preparation
2
Bacterial Growth Media Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For pre-cultures of the bacteria the basic buffer recipe was 10g/L yeast extract (Becton Dickinson, Franklin Lakes, USA) and 10g/L soytone (Becton Dickinson, Franklin Lakes, USA). We refer to that buffer as 1xNutrient medium (also 1xNu). The initial pH was 7 and 100mM phosphate were added. For the washing steps and the experiments itself the medium contained 1g/L yeast extract and 1g/L soytone, 0.1mM CaCl2, 2mM MgCl2, 4mg/L NiSO4, 50mg/L MnCl2 and 1x Trace Element Mix (Teknova, Hollister, USA). We refer to that buffer as base buffer. It was supplemented with phosphate buffer and/or glucose as outlined in the single experiments. The usual concentration was 10g/L glucose, deviations from that are described for the single experiments below. All media were filter sterilized.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!