Anti rock1 antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-ROCK1 antibody is a laboratory research tool used to detect and study the expression of the ROCK1 protein. ROCK1 is a serine/threonine protein kinase that plays a role in regulating cell shape and motility. This antibody can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and localization of ROCK1 in biological samples.

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Lab products found in correlation

Anti ve cadherin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh antibody, by Proteintech (1 mentions) Anti α sma antibody, by Merck Group (1 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 546 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Snail, by Santa Cruz Biotechnology (1 mentions) Rpmi 1640 medium, by American Type Culture Collection (1 mentions) Anti diap1 antibody, by Cell Signaling Technology (1 mentions) Anti lc3a b antibody, by Cell Signaling Technology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) L histidine, by Merck Group (1 mentions) Heat inactivated fetal bovine serum hi fbs, by Thermo Fisher Scientific (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Anti p62, by Cell Signaling Technology (1 mentions) Anti yap1, by Cell Signaling Technology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Anti bcl 2 antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-ROCK1 (26 citations) ROCK1 (19 citations)

3 protocols using anti rock1 antibody

Immunofluorescence Antibody Protocols

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Anti-VE-cadherin, CD31, and Snail antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-α-SMA antibody was obtained from Sigma-Aldrich (St. Louis, MO). Anti-p-MYPT1and anti-MYPT1 antibodies were obtained from Millipore (Billerica, MA). An anti-ROCK1 antibody was obtained from Cell Signaling Technology (CST, Danvers, MA). Alexa Fluor 546 anti-rabbit IgG and Alexa Fluor 488 anti-mouse IgG antibodies were obtained from Invitrogen (Carlsbad, CA). An anti-GAPDH antibody was obtained from Proteintech Group (PTG, Chicago, IL). Peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Boster (Wuhan, Hubei).

Peng H., Li Y., Wang C., Zhang J., Chen Y., Chen W., Cao J., Wang Y., Hu Z, & Lou T. (2016). ROCK1 Induces Endothelial-to-Mesenchymal Transition in Glomeruli to Aggravate Albuminuria in Diabetic Nephropathy. Scientific Reports, 6, 20304.

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Evaluating Cellular Responses to Oxidative Stress

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UM cells MEL270 HEK 293 cells and MCF-7 and RPMI-1640 medium (30–2001) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Heat-inactivated fetal bovine serum (HI-FBS) (10438–026) and Penicillin-Streptomycin (15140122) were purchased from Life Technologies (Grand Island, NY). Anti-Rock1 antibody (rabbit-4035), anti-Diap1 antibody (rabbit-5486), and anti-p62 (rabbit-5114), anti-YAP1 (rabbit-4912), anti-β-actin antibody (rabbit-2128), anti-LC3A/B antibody was (rabbit-4108) and HRP-linked secondary antibodies- anti rabbit (7074S, 7076S) were obtained from Cell Signaling Technology (Danvers, MA), anti-TEF1 was purchased by Abcam (rabbit-ab133533). Methionine sulfoxide antibody cat #600160 was from Cayman, USA. N-acetyl-L-cysteine and L-histidine were purchased from Sigma-Aldrich (A7250 and H8000 respectively). Verteporfin (Visudyne) was obtained from Novartis (Novartis, Basel, Switzerland) and was dissolved following the manufacture’s protocol.

Konstantinou E.K., Notomi S., Kosmidou C., Brodowska K., Al-Moujahed A., Nicolaou F., Tsoka P., Gragoudas E., Miller J.W., Young L.H, & Vavvas D.G. (2017). Verteporfin-induced formation of protein cross-linked oligomers and high molecular weight complexes is mediated by light and leads to cell toxicity. Scientific Reports, 7, 46581.

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Transfection and Western Blot Analysis

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The T24 and 5,637 cell lines were transfected with miR-148a-3p mimics, the miR-148a-3p inhibitor, negative control, and inhibitor NC using the methods above. 48 h after transfection, 1ml of RIPA buffer (Beyotime, China), 10 ul of protease inhibitor, and 10ul of phosphorylase inhibitor were used for total protein extraction and determination of protein density. The efficacy of transfection was tested by western blotting. The primary antibodies were as follow: anti-ROCK-1 antibody (1:1,000, Cell Signaling Technology, Danvers, MA, USA) and anti-Bcl-2 antibody (1:1,000, Cell Signaling Technology, Danvers, MA, USA). GAPDH was used as an endogenous control for normalization of the data.

Xu C., Zhou G., Sun Z., Zhang Z., Zhao H, & Jiang X. (2022). miR-148a-3p inhibits the proliferation and migration of bladder cancer via regulating the expression of ROCK-1. PeerJ, 10, e12724.

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