Agilent 1100 series lc msd trap mass spectrometer

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1100 Series LC/MSD Trap mass spectrometer is a laboratory instrument designed for the analysis of chemical compounds. It combines liquid chromatography (LC) with ion trap mass spectrometry (MSD Trap) to provide high-performance separation and detection capabilities. The core function of this system is to identify and quantify various molecules within a sample by measuring their mass-to-charge ratios.

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Lab products found in correlation

Agilent 1200 lc system, by Agilent Technologies (1 mentions) Agilent chemstation, by Agilent Technologies (1 mentions) Inova 500, by Agilent Technologies (1 mentions) 815 cd spectrometer, by Jasco (1 mentions) Pack ods a column, by YMC (1 mentions) Agilent 6520 accurate mass q tof lc ms spectrometer, by Agilent Technologies (1 mentions) Si gel, by Qingdao Marine Chemical (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) Silica gel, by Qingdao Marine Chemical (1 mentions) Acf 500 spectrometer, by Bruker (1 mentions)

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Agilent 1100 series (451 citations) 1100 series (251 citations) Agilent 1100 Series LC (23 citations) Agilent LC 1100 (21 citations) LC: 1100 series (20 citations) 1100 LC/MSD (18 citations) Agilent 1100 Series LC/MSD Trap (7 citations) 1100 Series LC/MSD Trap (7 citations) 1100 Series LC/MSD spectrometer (6 citations) LC-MSD 1100 series (3 citations)

5 protocols using agilent 1100 series lc msd trap mass spectrometer

Quantification of Glucosinolates and Flavonols

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Methanol extracts, prepared as described above, were used for the quantification of glucosinolates and flavonols in the samples (Section 2.4.1). 1ml of each extract was filtered using a 0.22 μm syringe driven filter unit (Millex; EMD Millipore, Billerica, MA, USA) and then diluted using 9ml LC-MS grade water. For the quantification of glucosinolates and flavonols, external calibration curves of 12 mM sinigrin hydrate and isorhamnetin standards were prepared using the following concentrations (56 ng.μl -1 , 42 ng.μl -1 , 28 ng.μl -1 , 14 ng.μl -1 , 5.6 ng.μl -1 , R 2 > 0.99).
Glucosinolates and flavonols were analysed by LC-MS/MS using an Agilent 1200
LC system coupled to an Agilent 1100 series LC/MSD mass trap spectrometer.
Separation conditions of samples and MS analysis settings used are identical to those described by Bell, et al. (2015) . Glucosinolates were quantified at 229 nm and flavonols at 330 nm. The identification was performed using the compounds nominal mass and the analysis of their fragmentation patterns, and also by the comparison with previously published data. All data were analysed using Agilent ChemStation.

Giallourou N., Oruna-Concha M.J, & Harbourne N. (2016). Effects of domestic processing methods on the phytochemical content of watercress (Nasturtium officinale). Food chemistry, 212.

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LC-MS Analysis of Seaweed Capsules and Biological Samples

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LC-MS analysis was conducted to analyse the food-grade seaweed capsule, the urine samples and the digested materials, and it was carried out in the negative ion mode using LC-MS/MS utilising electrospray ionisation (ESI), as previously described (48) . Characterisation was achieved using LC-MS/MS utilising ESI. This consisted of an Agilent 1200 HPLC system equipped with a binary pump, degasser, auto-sampler, thermostat, column heater, photodiode array detector and an Agilent 1100 series LC/MSD Mass Trap Spectrometer (Agilent Technologies UK). Separation of samples was achieved using a Zorbax SB C18 column

Corona G., Ji Y., Anegboonlap P., Hotchkiss S., Gill C., Yaqoob P., Spencer J.P, & Rowland I. (2016). Gastrointestinal modifications and bioavailability of brown seaweed phlorotannins and effects on inflammatory markers. The British journal of nutrition, 115(7).

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Spectroscopic Analysis of Organic Compounds

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Optical rotations were measured on a PE model 343 polarimeter (PerkinElmer, Waltham, MA, USA). CD spectra were recorded on a JASCO-815 CD spectrometer (JASCO, Tokyo, Japan). IR spectra were recorded on a Nicolet 5700 FT-IR microscope instrument (Thermo Fisher Scientific, Waltham, MA, USA). NMR spectra were obtained on INOVA-500 (Varian, Palo Alto, CA, USA) and Bruker AV600-III (Bruker, Billerica, MA, USA) spectrometers, in C₅D₅N with solvent peaks used as references. ESIMS were measured on an Agilent 1100 Series LC/MSD Trap mass spectrometer (Agilent, Santa Clara, CA, USA). HR-ESIMS data were measured using an Agilent 6520 Accurate-Mass Q-TOF LC/MS spectrometer (Agilent). Preparative HPLC was performed on a Shimadazu LC-6AD instrument with SPD-20A and RID-10A detectors (Shimadazu, Kyoto, Japan) using an YMC Pack ODS-A column (250 mm × 20 mm, 5 μm, YMC, Kyoto, Japan). Macroporous resin (D101 type, the Chemical Plant of NanKai University, Tianjin, China), Si gel (160–200 and 200–300 mesh, Qingdao Marine Chemical Factory, China) was used for column chromatography (CC). TLC was carried out with glass precoated Si gel GF₂₅₄ plates (Qingdao Marine Chemical Factory). Spots were visualized under UV light or by spraying with 10% H₂SO₄ in EtOH/H₂O (95:5, v/v) followed by heating.

Li Y., Zhu Y., Zhang Z., Li L., Liu Y., Qu J., Ma S, & Yu S. (2019). Antinociceptive grayanane-derived diterpenoids from flowers of Rhododendron molle. Acta Pharmaceutica Sinica. B, 10(6), 1073-1082.

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Synthesis and Characterization of Emodin

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The starting materials and reagents, purchased from commercial suppliers, were used without further purification. Emodin extracted from Polygonum cuspidatum was purchased from China Xi’an Sino-Herb Bio-technology Co., Ltd. (Xi’an, China). All reactions were monitored by thin-layer chromatography (TLC) on aluminum sheets (Silica gel 60-F254, E. Merck, Darmstadt, Germany). Compounds were visualized by UV light. Column chromatography was carried out using silica gel (200–300 mesh). All reaction solvents were dried prior to use according to standard procedures. All primary reagents were commercially available. Silica gel chromatography solvents were of analytical grade. NMR spectra were recorded in DMSO-d₆ on a Bruker-250 spectrometer (Bruker Biospin, Fällanden, Switzerlahd), at 400 MHz for ¹H-NMR, 101 MHz for ¹³C-NMR and 376 MHz for ¹⁹F-NMR with TMS as the internal standard. Chemical shifts were expressed in δ (ppm) and coupling constants (J) in Hz. Multiplicity was indicated as follows: s (singlet), d (doublet), t (triplet), p (quintet), dd (doublet of doublets), brs (broad singlet), etc. Mass spectra were obtained on an Agilent 1100 Series LC/MSD Trap mass spectrometer (ESI-MS, Agilent, Santa Clara, CA, USA).

Yang K., Jin M.J., Quan Z.S, & Piao H.R. (2019). Design and Synthesis of Novel Anti-Proliferative Emodin Derivatives and Studies on their Cell Cycle Arrest, Apoptosis Pathway and Migration. Molecules, 24(5), 884.

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Synthesis and Characterization of Novel Compounds

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All chemicals (reagent grade) used were purchased from Sino pharm Chemical Reagent Co., Ltd. (China). Reaction progress was monitored using analytical thin layer chromatography (TLC) on precoated silica gel GF254 (Qingdao Haiyang Chemical Plant, Qing-Dao, China) plates and the spots were detected under UV light (254 nm). Column chromatography was performed on silica gel (90–150 μm; Qingdao Marine Chemical Inc.). ¹H NMR and ¹³C NMR spectra were measured on a Bruker ACF-500 spectrometer at 25 °C and referenced to TMS. Chemical shifts are reported in ppm (δ) using the residual solvent line as internal standard. Splitting patterns are designed as s, singlet; d, doublet; t, triplet; m, multiplet. Mass spectra were obtained on a MS Agilent 1100 Series LC/MSD Trap mass spectrometer (ESI-MS).

Yang H., Jia H., Deng M., Zhang K., Liu Y., Liu Y., Cheng M, & Xiao W. (2023). Design, synthesis and evaluation of OA-tacrine hybrids as cholinesterase inhibitors with low neurotoxicity and hepatotoxicity against Alzheimer’s disease. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2192439.

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