Western blot analysis of total cell lysates (30 μg) was performed as previously described [30 (link)], using the following antibodies: anti-KLF7 (sc-101,034, Santa Cruz, Santa Cruz, CA, USA, 1:1000); anti-SNAIL (#3879, Cell Signaling Technology Inc., Danvers, MA, 1:1000); anti-ZEB2 (ab25837, Abcam, Cambridge, UK, 1:1000); anti-VIM monoclonal antibody (sc-73,259, Santa Cruz, 1:1000); anti-CD44 (M7082, Dako Agilent Technologies, Santa Clara, CA, USA, 1:500); anti-GAPDH (ab8245, Abcam, 1:5000). Blots were visualized by enhanced chemiluminescence system (Amersham Biosciences, Buckinghamshire, UK) using a Chemidoc imaging system (Bio-Rad). Experiments were performed at least three times. Proteins were densitometrically quantified using Imager ChemiDoc™ XRS+ Software (Bio-Rad, Version 6.0.1.34). All proteins were normalized to GAPDH loading control.
Anti klf7
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-KLF7 is a laboratory reagent that can be used to detect the presence and quantify the expression levels of the Krüppel-like factor 7 (KLF7) protein in various biological samples. KLF7 is a transcription factor involved in the regulation of cellular processes such as proliferation, differentiation, and apoptosis. The anti-KLF7 reagent can be utilized in techniques like Western blotting, immunohistochemistry, and ELISA to study the expression and localization of KLF7 in research settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti vps35, by Abcam (2 mentions)
Anti ccdc85c, by OriGene (2 mentions)
Anti β actin, by Santa Cruz Biotechnology (2 mentions)
Anti active β catenin, by Cell Signaling Technology (2 mentions)
Chemidoc imaging system, by Bio-Rad (1 mentions)
Anti cd44, by Agilent Technologies (1 mentions)
Enhanced chemiluminescence system, by Cytiva (1 mentions)
Anti zeb2, by Abcam (1 mentions)
Anti snail, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Anti gapdh 60004 1 ig, by Proteintech (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti klf7
1
Western Blot Analysis of Key Epithelial-Mesenchymal Transition Markers
2
Western Blot Analysis of HCC Cell Lines
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For western blot assay, HCC cell lines after transfection or without processing were harvested in lysis buffer. The concentration of total protein was evaluated by BCA protein assay kit (Thermo Fisher Scientific, lnc.). Then the protein was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Thermo Fisher Scientific, lnc.). After incubation with 5% milk at room temperature for 2 h, the membranes were maintained with primary antibody as indicated at 4 ℃ overnight and then incubated with secondary antibodies at room temperature for 2 h. Protein bands were visualized with an enhanced chemiluminescence (ECL) assay. (Millipore, Billerica, MA). GAPDH was acted as endogenous control. The primary antibodies used as follow: anti-KLF7 (398576, Santa Cruz), anti-GAPDH (60004–1-Ig, Proteintech), anti-VPS35 (157220, Abcam), anti-CCDC85C (TA330857, ORIGENE), anti-active β-catenin (19807, Cell Signaling Technology), anti-β-actin (47778, Santa Cruz).
3
Western Blot Assay for HCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For western blot assay, HCC cell lines after transfection or without processing were harvested in lysis buffer. The concentration of total protein was evaluated by BCA protein assay kit (Thermo Fisher Scienti c, lnc.). Then the protein was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Thermo Fisher Scienti c, lnc.). After incubation with 5% milk at room temperature for 2 h, the membranes were maintained with primary antibody as indicated at 4 ℃ overnight and then incubated with secondary antibodies at room temperature for 2 h. Protein bands were visualized with an enhanced chemiluminescence (ECL) assay. (Millipore, Billerica, MA). GAPDH was acted as endogenous control. The primary antibodies used as follow: anti-KLF7 (398576, Santa Cruz), anti-GAPDH (60004-1-Ig, Proteintech), anti-VPS35 (157220, Abcam), anti-CCDC85C (TA330857, ORIGENE), anti-active β-catenin (19807, Cell Signaling Technology), anti-β-actin (47778, Santa Cruz).
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