Christ alpha 2 4ld plus freeze dryer

TH-NPs were prepared using the single emulsion-solvent evaporation method previously described with slight modifications [15 (link)]. Briefly, 75 mg of PLGA and 7.5 mg of thymol were dissolved in 1.5 mL of dichloromethane. Then, 3 mL of polyvinyl alcohol solution prepared in distilled water at 2% (W/V) was added dropwise into the organic phase and magnetically stirred for 3 h to evaporate dichloromethane. The solution was centrifuged at 65,000 ×g for 20 min, and the obtained suspension was washed thrice with distilled water. The TH-NP sample suspension obtained was freeze-dried using a Christ Alpha 2–4 LDplus freeze dryer (Christ, Osterode am Harz, Germany) and stored at −20°C for further analysis.
Dynamic light scattering (DLS) was used to measure the particle size, polydispersity index (PDI), and zeta potential of the obtained nanoparticles.

In this study, raw Polish plants from the Alliaceae family—i.e., garlic (Allim sativum L.) (“Harnaś” cultivar), white and red onion (Allium cepa L.) (“Amstrong” and “Red Baron” cultivar, respectively)—were collected from the test plots, which were donated by the Polish company Elena (Kokanin, Żelazków, Poland). The bulbs were washed, cleaned, peeled, cut into small, equal sized pieces and weighed. After that, the samples were frozen in liquid nitrogen and then freeze-dried in a freeze dryer (CHRIST ALPHA 2-4 LDplus Freeze Dryer, Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany), at a chamber pressure of 10 Pa, a drying chamber temperature of −50 °C, and a shelf temperature of 21 °C for 72 h. The freeze-dried material was weighed, ground to a powder in a laboratory knife mill (Grindomix GM 200, Retsch GmbH, Haan, Germany), packed into Falcone tubes and stored in freezing conditions at −20 °C until the commencement of analysis and animal study. Lyophilized material was used for testing the content of selected bioactive ingredients, their antioxidant potential, and for the animal studies.