Agilent 1200 series diode array

Optical rotations ( ${[\alpha ]}_{\mathrm{D}}^{25}$ ) were measured on an Anton Paar MCP 200 Modular Circular Polarimeter (Anton Paar GmbH, Graz, Austria) in a 100 × 2 mm cell. CD spectra were recorded on an Applied Photophysics Chirascan spectropolarimeter (Applied Photophysics Ltd., Leatherhead, UK). NMR spectra were obtained on a Bruker Avance 500 spectrometer (Bruker BioSpin Corp., Billerica, MA, USA) with residual solvent peaks as references (DMSO-d₆: δ_H 2.50, δ_C 39.52; acetone-d₆: δ_H 2.05, δ_C 29.84; CDCl₃: δ_H 7.26, δ_C 77.16). High-resolution ESIMS measurements were obtained on an Accurate-Mass-Q-TOF LC/MS 6520 instrument (Agilent Technologies, Santa Clara, CA, USA) in positive ion mode. HPLC was performed using an Agilent 1200 Series separation module equipped with an Agilent 1200 Series diode array and Agilent 1260 Series fraction collector (Agilent Technologies, Santa Clara, CA, USA), and an Agilent ZORBAX SB-C18 column (250 × 9.4 mm, 5 µm).

Optical rotations ([α $${]}_D^{25}$$ ) were measured on an Anton Paar MCP 200 Modular Circular Polarimeter (Austria) in a 100 × 2 mm cell. CD spectra were recorded on an Applied Photophysics Chirascan spectropolarimeter (Surrey, UK). NMR spectra were obtained on a Bruker Avance 500 spectrometer with residual solvent peaks as references (DMSO-d₆: δ_H 2.50, δ_C 39.52). High-resolution ESIMS measurements were obtained on an Accurate-Mass-Q-TOF LC/MS 6520 instrument (Santa Clara, CA, USA) in the positive ion mode. HPLC was performed using an Agilent 1200 Series separation module equipped with an Agilent 1200 Series diode array, Agilent 1260 Series fraction collector, and Agilent ZORBAX SB-C18 column (250 × 9.4 mm, 5 µm).